2JEA

Structure of a 9-subunit archaeal exosome bound to RNA

2JEA の概要

• エントリーDOI: 10.2210/pdb2jea/pdb
• 関連するPDBエントリー: 2BR2 2C37 2C38 2C39 2JE6 2JEB
• 分子名称: EXOSOME COMPLEX EXONUCLEASE 2, EXOSOME COMPLEX EXONUCLEASE 1, RNA, ... (6 entities in total)
• 機能のキーワード: hydrolase rna complex, hydrolase-rna complex, nuclease, hydrolase, rna-binding, exonuclease, phosphorolytic, exoribonuclease, rna degradation, rrp4, rrp42, rrp41, exosome, rnase ph, hydrolase/rna
• 由来する生物種: SULFOLOBUS SOLFATARICUS; 詳細
• 細胞内の位置: Cytoplasm (Potential): Q9UXC0 2JEA Q9UXC2
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 98079.60

構造登録者

Lorentzen, E.,Conti, E. (登録日: 2007-01-16, 公開日: 2007-04-03, 最終更新日: 2023-12-13)

主引用文献

Lorentzen, E.,Dziembowski, A.,Lindner, D.,Seraphin, B.,Conti, E.
RNA Channelling by the Archaeal Exosome.
Embo Rep., 8:470-, 2007

PubMed Abstract: Exosomes are complexes containing 3' --> 5' exoribonucleases that have important roles in processing, decay and quality control of various RNA molecules. Archaeal exosomes consist of a hexameric core of three active RNase PH subunits (ribosomal RNA processing factor (Rrp)41) and three inactive RNase PH subunits (Rrp42). A trimeric ring of subunits with putative RNA-binding domains (Rrp4/cep1 synthetic lethality (Csl)4) is positioned on top of the hexamer on the opposite side to the RNA degrading sites. Here, we present the 1.6 A resolution crystal structure of the nine-subunit exosome of Sulfolobus solfataricus and the 2.3 A structure of this complex bound to an RNA substrate designed to be partly trimmed rather than completely degraded. The RNA binds both at the active site on one side of the molecule and on the opposite side in the narrowest constriction of the central channel. Multiple substrate-binding sites and the entrapment of the substrate in the central channel provide a rationale for the processive degradation of extended RNAs and the stalling of structured RNAs.

PubMed: 17380186
DOI: 10.1038/SJ.EMBOR.7400945

実験手法

X-RAY DIFFRACTION (2.33 Å)