2JDH

Lectin PA-IIL of P.aeruginosa complexed with disaccharide derivative

2JDH の概要

• エントリーDOI: 10.2210/pdb2jdh/pdb
• 関連するPDBエントリー: 1GZT 1OUR 1OUS 1OUX 1OVP 1OVS 1OXC 1UZV 1W43 1W8F 1W8H 2BOJ 2BP6
• 分子名称: FUCOSE-BINDING LECTIN PA-IIL, alpha-L-fucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-L-fucopyranose, ... (7 entities in total)
• 機能のキーワード: lectin, cystic fibrosis, sugar binding protein
• 由来する生物種: PSEUDOMONAS AERUGINOSA
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 49443.78

構造登録者

Marotte, K.,Sabin, C.,Preville, C.,Pymbock, M.,Deguise, I.,Wimmerova, M.,Mitchell, E.P.,Imberty, A.,Roy, R. (登録日: 2007-01-09, 公開日: 2007-07-24, 最終更新日: 2023-12-13)

主引用文献

Marotte, K.,Sabin, C.,Preville, C.,Pymbock, M.,Wimmerova, M.,Mitchell, E.P.,Imberty, A.,Roy, R.
X-Ray Structures and Thermodynamics of the Interaction of Pa-Iil from Pseudomonas Aeruginosa with Disaccharide Derivatives.
Chemmedchem, 2:1328-, 2007

PubMed Abstract: Pseudomonas aeruginosa is an opportunistic bacterium showing increasing resistance to antibiotics and consequently represents elevated threatening problems in hospital environments, particularly for cystic fibrosis patients. The use of glycomimetics as an anti-adhesive strategy against microorganisms may complement the use of antibiotics. PA-IIL lectin (LecB) from P. aeruginosa constitutes an appealing target for antibacterial agents, as it has been proposed to play a key role in binding to airway epithelia and/or to be involved in biofilm formation. The lectin has an unusually high affinity for L-fucose and related oligosaccharides. In the work presented herein, the disaccharide alphaFuc1-4GlcNAc is used as a scaffold toward the synthesis of a series of glycomimetic derivatives. Microcalorimetry and structural studies indicate that several of the derivatives are potent inhibitors of the lectin, with affinity in the same range as the best known natural ligand, Lewis a, and could represent interesting leads for the development of future antibacterial compounds.

PubMed: 17623286
DOI: 10.1002/CMDC.200700100

実験手法

X-RAY DIFFRACTION (1.1 Å)