2JD5

Sky1p bound to Npl3p-derived substrate peptide

2JD5 の概要

• エントリーDOI: 10.2210/pdb2jd5/pdb
• 関連するPDBエントリー: 1HOW 1Q8Y 1Q8Z 1Q97 1Q99
• 分子名称: SERINE/THREONINE-PROTEIN KINASE SKY1, NUCLEOLAR PROTEIN 3, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: mrna export protein, serine/threonine-protein kinase, kinase, transferase, atp-binding, nucleotide-binding
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); 詳細
• 細胞内の位置: Nucleus, nucleolus : Q01560
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 87162.88

構造登録者

Nolen, B.,Lukasiewicz, R.,Adams, J.A.,Huang, D.,Ghosh, G. (登録日: 2007-01-04, 公開日: 2007-02-06, 最終更新日: 2023-12-13)

主引用文献

Lukasiewicz, R.,Nolen, B.,Adams, J.A.,Ghosh, G.
The Rgg Domain of Npl3P Recruits Sky1P Through Docking Interactions
J.Mol.Biol., 367:249-, 2007

PubMed Abstract: The SR protein kinase in yeast, Sky1p, phosphorylates yeast SR-like protein, Npl3p, at a single serine residue located at its C terminus. We report here the X-ray crystal structure of Sky1p bound to a substrate peptide and ADP. Surprisingly, an Npl3p-derived substrate peptide occupies a groove 20 A away from the kinase active site. In vitro studies support the substrate-docking role of this groove. Mutagenesis and binding studies reveal that multiple degenerate short peptide motifs located within the RGG domain of Npl3p serve as the substrate docking motifs. However, a single docking motif is sufficient for its stable interaction with the kinase. Methylation of the docking motifs abolishes kinase binding and phosphorylation of Npl3p. Remarkably, removal of the docking groove in the kinase or the docking motifs of the substrate does not reduce the overall catalytic efficiency of the phosphorylation reaction in any significant manner. We suggest that docking interaction between Sky1p and Npl3p is essential for substrate recruitment and binding specificity.

PubMed: 17239901
DOI: 10.1016/J.JMB.2006.12.031

実験手法

X-RAY DIFFRACTION (2.5 Å)