2JAT

Structure of deoxyadenosine kinase from M.mycoides with products dcmp and a flexible dcdp bound

2JAT の概要

• エントリーDOI: 10.2210/pdb2jat/pdb
• 関連するPDBエントリー: 2JAQ 2JAS
• 分子名称: DEOXYGUANOSINE KINASE, MAGNESIUM ION, PYROPHOSPHATE 2-, ... (5 entities in total)
• 機能のキーワード: kinase, transferase, deoxyribonucleoside kinase
• 由来する生物種: MYCOPLASMA MYCOIDES SUBSP. MYCOIDES SC
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49592.63

構造登録者

Welin, M.,Wang, L.,Eriksson, S.,Eklund, H. (登録日: 2006-11-30, 公開日: 2007-01-03, 最終更新日: 2023-12-13)

主引用文献

Welin, M.,Wang, L.,Eriksson, S.,Eklund, H.
Structure-Function Analysis of a Bacterial Deoxyadenosine Kinase Reveals the Basis for Substrate Specificity.
J.Mol.Biol., 366:1615-, 2007

PubMed Abstract: Deoxyribonucleoside kinases (dNKs) catalyze the transfer of a phosphoryl group from ATP to a deoxyribonucleoside (dN), a key step in DNA precursor synthesis. Recently structural information concerning dNKs has been obtained, but no structure of a bacterial dCK/dGK enzyme is known. Here we report the structure of such an enzyme, represented by deoxyadenosine kinase from Mycoplasma mycoides subsp. mycoides small colony type (Mm-dAK). Superposition of Mm-dAK with its human counterpart's deoxyguanosine kinase (dGK) and deoxycytidine kinase (dCK) reveals that the overall structures are very similar with a few amino acid alterations in the proximity of the active site. To investigate the substrate specificity, Mm-dAK has been crystallized in complex with dATP and dCTP, as well as the products dCMP and dCDP. Both dATP and dCTP bind to the enzyme in a feedback-inhibitory manner with the dN part in the deoxyribonucleoside binding site and the triphosphates in the P-loop. Substrate specificity studies with clinically important nucleoside analogs as well as several phosphate donors were performed. Thus, in this study we combine structural and kinetic data to gain a better understanding of the substrate specificity of the dCK/dGK family of enzymes. The structure of Mm-dAK provides a starting point for making new anti bacterial agents against pathogenic bacteria.

PubMed: 17229440
DOI: 10.1016/J.JMB.2006.12.010

実験手法

X-RAY DIFFRACTION (2.6 Å)