2JAK

Human PP2A regulatory subunit B56g

2JAK の概要

• エントリーDOI: 10.2210/pdb2jak/pdb
• 分子名称: SERINE/THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT GAMMA ISOFORM (2 entities in total)
• 機能のキーワード: b56g, pp2a, ppp2r5c, phosphorylation, pp2a regulatory subunit, nuclear protein
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Nucleus: Q13362
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45859.86

構造登録者

Magnusdottir, A.,Stenmark, P.,Arrowsmith, C.,Berglund, H.,Busam, R.,Collins, R.,Edwards, A.,Ericsson, U.B.,Flodin, S.,Flores, A.,Graslund, S.,Hammarstrom, M.,Hallberg, B.M.,Holmberg Schiavone, L.,Hogbom, M.,Johansson, I.,Karlberg, T.,Kotenyova, T.,Lundgren, S.,Moche, M.,Nilsson, P.,Nyman, T.,Ogg, D.,Persson, C.,Sagemark, J.,Sundstrom, M.,Uppenberg, J.,Upsten, M.,Thorsell, A.G.,Van Den Berg, S.,Weigelt, J.,Nordlund, P. (登録日: 2006-11-29, 公開日: 2006-12-04, 最終更新日: 2024-05-08)

主引用文献

Magnusdottir, A.,Stenmark, P.,Flodin, S.,Nyman, T.,Kotenyova, T.,Graslund, S.,Ogg, D.,Nordlund, P.
The Structure of the Pp2A Regulatory Subunit B56 Gamma: The Remaining Piece of the Pp2A Jigsaw Puzzle.
Proteins, 74:212-, 2009

PubMed Abstract: The PP2A serine/threonine phosphatase regulates a plethora of cellular processes. In the cell the predominant form of the enzyme is a heterotrimer, formed by a core dimer composed of a catalytic and a scaffolding subunit, which assemble together with one of a range of different regulatory B subunits. Here, we present the first structure of a free non-complexed B subunit, B56 gamma. Comparison with the recent structures of a heterotrimeric complex and the core dimer reveals several significant conformational changes in the interface region between the B56 gamma and the core dimer. These allow for an assembly scheme of the PP2A holoenzyme to be put forth where B56 gamma first complexes with the scaffolding subunit and subsequently binds to the catalytic subunit and this induces the formation of a binding site for the invariant C-terminus of the catalytic subunit that locks in the complex as a last step of assembly.

PubMed: 18618707
DOI: 10.1002/PROT.22150

実験手法

X-RAY DIFFRACTION (2.6 Å)