2J96

The E-configuration of alfa-Phycoerythrocyanin

2J96 の概要

• エントリーDOI: 10.2210/pdb2j96/pdb
• 関連するPDBエントリー: 2C7J 2C7K 2C7L
• 分子名称: PHYCOERYTHROCYANIN ALPHA CHAIN, PHYCOVIOLOBILIN (3 entities in total)
• 機能のキーワード: electron transport, z- to e-isomerization, transport, chromophore, bile pigment, phycobilisome, photosynthesis, light harvesting, phycobiliproteins
• 由来する生物種: MASTIGOCLADUS LAMINOSUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36340.76

構造登録者

Schmidt, M.,Patel, A.,Zhao, Y.,Reuter, W. (登録日: 2006-11-02, 公開日: 2007-01-23, 最終更新日: 2024-11-20)

主引用文献

Schmidt, M.,Patel, A.,Zhao, Y.,Reuter, W.
Structural Basis for the Photochemistry of Alfa-Phycoerythrocyanin
Biochemistry, 46:416-, 2007

PubMed Abstract: Phycobiliproteins and phytochromes are light-harvesting and light-sensing proteins containing linear tetrapyrroles, so-called bile chromophores. The chromophores in certain biliproteins, including the phytochromes, isomerize reversibly from a stable Z-configuration to a stable E-configuration when irradiated with light of the appropriate wavelength. Here, we report the crystal structure of alpha-phycoerythrocyanin with its chromophore in the E-configuration, compare it with the Z-configuration found in trimeric phycoerythrocyanin, and reveal the structural bases of the isomerization. The geometric changes of the chromophore account for the large spectral shift, which characterizes the overall transition. Interactions of the chromophore A and D pyrrole rings with flexible protein moieties are required for the formation and stabilization of the isomers. We predict that the results will hold for all photoactive biliproteins.

PubMed: 17209552
DOI: 10.1021/BI061844J

実験手法

X-RAY DIFFRACTION (2.25 Å)