2J56

X-ray reduced Paraccocus denitrificans methylamine dehydrogenase N- semiquinone in complex with amicyanin.

2J56 の概要

• エントリーDOI: 10.2210/pdb2j56/pdb
• 関連するPDBエントリー: 1AAC 1AAJ 1AAN 1BXA 1MDA 1MG2 1MG3 1SF3 1SF5 1SFD 1SFH 1T5K 2BBK 2J55 2J57 2MTA 2RAC
• 分子名称: AMICYANIN, METHYLAMINE DEHYDROGENASE HEAVY CHAIN, METHYLAMINE DEHYDROGENASE LIGHT CHAIN, ... (7 entities in total)
• 機能のキーワード: oxidoreductase, periplasmic, metal-binding, electron transport, single crystal microspectrophotometry
• 由来する生物種: PARACOCCUS DENITRIFICANS; 詳細
• 細胞内の位置: Periplasm: P22364 P29894 P22619
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 136593.49

構造登録者

Pearson, A.R.,Pahl, R.,Davidson, V.L.,Wilmot, C.M. (登録日: 2006-09-12, 公開日: 2007-01-23, 最終更新日: 2011-07-13)

主引用文献

Pearson, A.R.,Pahl, R.,Kovaleva, E.G.,Davidson, V.L.,Wilmot, C.M.
Tracking X-Ray-Derived Redox Changes in Crystals of a Methylamine Dehydrogenase/Amicyanin Complex Using Single-Crystal Uv/Vis Microspectrophotometry.
J.Synchrotron Radiat., 14:92-, 2007

PubMed Abstract: X-ray exposure during crystallographic data collection can result in unintended redox changes in proteins containing functionally important redox centers. In order to directly monitor X-ray-derived redox changes in trapped oxidative half-reaction intermediates of Paracoccus denitrificans methylamine dehydrogenase, a commercially available single-crystal UV/Vis microspectrophotometer was installed on-line at the BioCARS beamline 14-BM-C at the Advanced Photon Source, Argonne, USA. Monitoring the redox state of the intermediates during X-ray exposure permitted the creation of a general multi-crystal data collection strategy to generate true structures of each redox intermediate.

PubMed: 17211075
DOI: 10.1107/S0909049506051259

実験手法

X-RAY DIFFRACTION (2.1 Å)