2J1K

CAV-2 fibre head in complex with CAR D1

2J1K の概要

• エントリーDOI: 10.2210/pdb2j1k/pdb
• 関連するPDBエントリー: 1KAC 1NOB 1P69 1P6A
• 分子名称: COXSACKIEVIRUS AND ADENOVIRUS RECEPTOR, FIBER PROTEIN (3 entities in total)
• 機能のキーワード: virus-receptor complex, complex, membrane, receptor, palmitate, domain d1, immunoglobulin domain, host-virus interaction, glycoprotein, fiber protein, cell adhesion, phosphorylation, transmembrane, tight junction, coxsackievirus, fiber head, adenovirus, fibre head, lipoprotein, car, knob, fiber, fibre, cav-2, canine, virus/receptor
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Single-pass type I membrane protein. Isoform 3: Secreted. Isoform 4: Secreted. Isoform 5: Secreted: P78310
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 431241.43

構造登録者

Seiradake, E.,Lortat-Jacob, H.,Billet, O.,Kremer, E.J.,Cusack, S. (登録日: 2006-08-14, 公開日: 2006-09-05, 最終更新日: 2023-12-13)

主引用文献

Seiradake, E.,Lortat-Jacob, H.,Billet, O.,Kremer, E.J.,Cusack, S.
Structural and Mutational Analysis of Human Ad37 and Canine Adenovirus 2 Fiber Heads in Complex with the D1 Domain of Coxsackie and Adenovirus Receptor.
J.Biol.Chem., 281:33704-, 2006

PubMed Abstract: Adenovirus fibers from most serotypes bind the D1 domain of coxsackie and adenovirus receptor (CAR), although the binding residues are not strictly conserved. To understand this further, we determined the crystal structures of canine adenovirus serotype 2 (CAV-2) and the human adenovirus serotype 37 (HAd37) in complex with human CAR D1 at 2.3 and 1.5A resolution, respectively. Structure comparison with the HAd12 fiber head-CAR D1 complex showed that the overall topology of the interaction is conserved but that the interfaces differ in number and identity of interacting residues, shape complementarity, and degree of conformational adaptation. Using surface plasmon resonance, we characterized the binding affinity to CAR D1 of wild type and mutant CAV-2 and HAd37 fiber heads. We found that CAV-2 has the highest affinity but fewest direct interactions, with the reverse being true for HAd37. Moreover, we found that conserved interactions can have a minor contribution, whereas serotype-specific interactions can be essential. These results are discussed in the light of virus evolution and design of adenovirus vectors for gene transfer.

PubMed: 16923808
DOI: 10.1074/JBC.M605316200

実験手法

X-RAY DIFFRACTION (2.3 Å)