2J18

Chloroperoxidase mixture of ferric and ferrous states (low dose data set)

2J18 の概要

• エントリーDOI: 10.2210/pdb2j18/pdb
• 関連するPDBエントリー: 1CPO 2CIV 2CIW 2CIX 2CIY 2CIZ 2CJ0 2CJ1 2CJ2 2CPO 2J19
• 関連するBIRD辞書のPRD_ID: PRD_900111
• 分子名称: CHLOROPEROXIDASE, alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (10 entities in total)
• 機能のキーワード: oxidoreductase, pyrrolidone carboxylic acid, glycoprotein, metal-binding, iron, heme, chloride, manganese, peroxidase
• 由来する生物種: CALDARIOMYCES FUMAGO
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36853.87

構造登録者

Beitlich, T.,Kuhnel, K.,Schulze-Briese, C.,Shoeman, R.L.,Schlichting, I. (登録日: 2006-08-09, 公開日: 2006-12-18, 最終更新日: 2024-11-20)

主引用文献

Beitlich, T.,Kuhnel, K.,Schulze-Briese, C.,Shoeman, R.L.,Schlichting, I.
Cryoradiolytic Reduction of Crystalline Heme Proteins: Analysis by Uv-Vis Spectroscopy and X-Ray Crystallography
J.Synchrotron Radiat., 14:11-, 2007

PubMed Abstract: The X-ray crystallographic analysis of redox-active systems may be complicated by photoreduction. Although radiolytic reduction by the probing X-ray beam may be exploited to generate otherwise short-lived reaction intermediates of metalloproteins, it is generally an undesired feature. Here, the X-ray-induced reduction of the three heme proteins myoglobin, cytochrome P450cam and chloroperoxidase has been followed by on-line UV-Vis absorption spectroscopy. All three systems showed a very rapid reduction of the heme iron. In chloroperoxidase the change of the ionization state from ferric to ferrous heme is associated with a movement of the heme-coordinating water molecule. The influence of the energy of the incident X-ray photons and of the presence of scavengers on the apparent reduction rate of ferric myoglobin crystals was analyzed.

PubMed: 17211068
DOI: 10.1107/S0909049506049806

実験手法

X-RAY DIFFRACTION (1.75 Å)