2IZO

Structure of an Archaeal PCNA1-PCNA2-FEN1 Complex

2IZO の概要

• エントリーDOI: 10.2210/pdb2izo/pdb
• 関連するPDBエントリー: 2IX2
• 分子名称: FLAP STRUCTURE-SPECIFIC ENDONUCLEASE, DNA POLYMERASE SLIDING CLAMP C, DNA POLYMERASE SLIDING CLAMP B, ... (6 entities in total)
• 機能のキーワード: hydrolase, dna repair, dna-binding, endonuclease, metal-binding, excision repair, dna replication, pcna, fen1, nuclease, magnesium
• 由来する生物種: SULFOLOBUS SOLFATARICUS; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 94838.13

構造登録者

Dore, A.S.,Kilkenny, M.L.,Roe, S.M.,Pearl, L.H. (登録日: 2006-07-25, 公開日: 2006-09-06, 最終更新日: 2023-12-13)

主引用文献

Dore, A.S.,Kilkenny, M.L.,Jones, S.A.,Oliver, A.W.,Roe, S.M.,Bell, S.D.,Pearl, L.H.
Structure of an Archaeal PCNA1-PCNA2-Fen1 Complex: Elucidating PCNA Subunit and Client Enzyme Specificity.
Nucleic Acids Res., 34:4515-, 2006

PubMed Abstract: The archaeal/eukaryotic proliferating cell nuclear antigen (PCNA) toroidal clamp interacts with a host of DNA modifying enzymes, providing a stable anchorage and enhancing their respective processivities. Given the broad range of enzymes with which PCNA has been shown to interact, relatively little is known about the mode of assembly of functionally meaningful combinations of enzymes on the PCNA clamp. We have determined the X-ray crystal structure of the Sulfolobus solfataricus PCNA1-PCNA2 heterodimer, bound to a single copy of the flap endonuclease FEN1 at 2.9 A resolution. We demonstrate the specificity of interaction of the PCNA subunits to form the PCNA1-PCNA2-PCNA3 heterotrimer, as well as providing a rationale for the specific interaction of the C-terminal PIP-box motif of FEN1 for the PCNA1 subunit. The structure explains the specificity of the individual archaeal PCNA subunits for selected repair enzyme 'clients', and provides insights into the co-ordinated assembly of sequential enzymatic steps in PCNA-scaffolded DNA repair cascades.

PubMed: 16945955
DOI: 10.1093/NAR/GKL623

実験手法

X-RAY DIFFRACTION (2.9 Å)