2IVK

Crystal structure of the periplasmic endonuclease Vvn complexed with a 16-bp DNA

2IVK の概要

• エントリーDOI: 10.2210/pdb2ivk/pdb
• 分子名称: ENDONUCLEASE I, 5'-D(*GP*AP*AP*TP*TP*CP*GP*AP*TP*CP *GP*AP*AP*TP*T)-3', 5'-D(*AP*AP*TP*TP*CP*GP*AP*TP*CP*GP *AP*AP*TP*TP*C)-3', ... (5 entities in total)
• 機能のキーワード: dnase, endonuclease, dna hydrolysis, protein nucleic acid interactions, dna cleavage preference, hydrolase
• 由来する生物種: VIBRIO VULNIFICUS; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 127153.28

構造登録者

Wang, Y.T.,Yang, W.J.,Li, C.L.,Doudeva, L.G.,Yuan, H.S. (登録日: 2006-06-14, 公開日: 2007-01-02, 最終更新日: 2024-11-13)

主引用文献

Wang, Y.T.,Yang, W.J.,Li, C.L.,Doudeva, L.G.,Yuan, H.S.
Structural Basis for Sequence-Dependent DNA Cleavage by Nonspecific Endonucleases.
Nucleic Acids Res., 35:584-, 2007

PubMed Abstract: Nonspecific endonucleases hydrolyze DNA without sequence specificity but with sequence preference, however the structural basis for cleavage preference remains elusive. We show here that the nonspecific endonuclease ColE7 cleaves DNA with a preference for making nicks after (at 3'O-side) thymine bases but the periplasmic nuclease Vvn cleaves DNA more evenly with little sequence preference. The crystal structure of the 'preferred complex' of the nuclease domain of ColE7 bound to an 18 bp DNA with a thymine before the scissile phosphate had a more distorted DNA phosphate backbone than the backbones in the non-preferred complexes, so that the scissile phosphate was compositionally closer to the endonuclease active site resulting in more efficient DNA cleavage. On the other hand, in the crystal structure of Vvn in complex with a 16 bp DNA, the DNA phosphate backbone was similar and not distorted in comparison with that of a previously reported complex of Vvn with a different DNA sequence. Taken together these results suggest a general structural basis for the sequence-dependent DNA cleavage catalyzed by nonspecific endonucleases, indicating that nonspecific nucleases could induce DNA to deform to distinctive levels depending on the local sequence leading to different cleavage rates along the DNA chain.

PubMed: 17175542
DOI: 10.1093/NAR/GKL621

実験手法

X-RAY DIFFRACTION (2.9 Å)