2ITL

The origin binding domain of the SV40 large T antigen bound to the functional pen palindrome DNA (23 bp)

2ITL の概要

• エントリーDOI: 10.2210/pdb2itl/pdb
• 関連するPDBエントリー: 2IPR 2ITJ 2NL8
• 分子名称: 24-nt PEN element of the SV40 DNA origin, large T antigen, ... (4 entities in total)
• 機能のキーワード: dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Simian virus 40
• 細胞内の位置: Host nucleus : P03070
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 45516.87

構造登録者

Bochkareva, E.,Seitova, A.,Bochkarev, A. (登録日: 2006-10-19, 公開日: 2006-12-12, 最終更新日: 2023-08-30)

主引用文献

Bochkareva, E.,Martynowski, D.,Seitova, A.,Bochkarev, A.
Structure of the origin-binding domain of simian virus 40 large T antigen bound to DNA
Embo J., 25:5961-5969, 2006

PubMed Abstract: The large T antigen (T-ag) protein binds to and activates DNA replication from the origin of DNA replication (ori) in simian virus 40 (SV40). Here, we determined the crystal structures of the T-ag origin-binding domain (OBD) in apo form, and bound to either a 17 bp palindrome (sites 1 and 3) or a 23 bp ori DNA palindrome comprising all four GAGGC binding sites for OBD. The T-ag OBDs were shown to interact with the DNA through a loop comprising Ser147-Thr155 (A1 loop), a combination of a DNA-binding helix and loop (His203-Asn210), and Asn227. The A1 loop traveled back-and-forth along the major groove and accounted for most of the sequence-determining contacts with the DNA. Unexpectedly, in both T-ag-DNA structures, the T-ag OBDs bound DNA independently and did not make direct protein-protein contacts. The T-ag OBD was also captured bound to a non-consensus site ATGGC even in the presence of its canonical site GAGGC. Our observations taken together with the known biochemical and structural features of the T-ag-origin interaction suggest a model for origin unwinding.

PubMed: 17139255
DOI: 10.1038/sj.emboj.7601452

実験手法

X-RAY DIFFRACTION (1.65 Å)