2ISA

Crystal Structure of Vibrio salmonicida catalase

2ISA の概要

• エントリーDOI: 10.2210/pdb2isa/pdb
• 分子名称: Catalase, PROTOPORPHYRIN IX CONTAINING FE, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: peroxidase, heme, oxidoreductase, iron, hydrogen peroxide
• 由来する生物種: Vibrio salmonicida
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 444569.59

構造登録者

Riise, E.K.,Lorentzen, M.S.,Helland, R.,Smalas, A.O.,Leiros, H.K.S.,Willassen, N.P. (登録日: 2006-10-17, 公開日: 2007-01-23, 最終更新日: 2024-10-16)

主引用文献

Riise, E.K.,Lorentzen, M.S.,Helland, R.,Smalas, A.O.,Leiros, H.K.S.,Willassen, N.P.
The first structure of a cold-active catalase from Vibrio salmonicida at 1.96A reveals structural aspects of cold adaptation
ACTA CRYSTALLOGR.,SECT.D, 63:135-148, 2007

PubMed Abstract: The cold-adapted catalase from the fish-pathogenic bacterium Vibrio salmonicida (VSC) has recently been characterized and shown to be two times more catalytically efficient compared with catalase from the mesophilic human pathogen Proteus mirabilis [PMC; Lorentzen et al. (2006), Extremophiles, 10, 427-440]. VSC is also less temperature-stable, with a half-life of 5 min at 333 K compared with 50 min for PMC. This was the background for solving the crystal structure of the cold-adapted VSC to 1.96 A and performing an extensive structural comparison of VSC and PMC. The comparison revealed that the entrance (the major channel) leading to the catalytically essential haem group, is locally more flexible and slightly wider in VSC. This might explain the enhanced catalytic efficiency of the nearly diffusion-controlled degradation of hydrogen peroxide into water and molecular oxygen in VSC. The reduced thermal stability of the cold-adapted VSC may be explained by a reduced number of ion-pair networks. The four C-terminal alpha-helices are displaced in the structures, probably owing to missing ionic interactions in VSC compared with PMC, and this is postulated as an initiation site for unfolding the cold-adapted enzyme. VSC is the first crystal structure reported of a cold-adapted monofunctional haem-containing catalase.

PubMed: 17242507
DOI: 10.1107/S0907444906043812

実験手法

X-RAY DIFFRACTION (1.97 Å)