2INY

Nanoporous Crystals of Chicken Embryo Lethal Orphan (CELO) Adenovirus Major Coat Protein, Hexon

2INY の概要

• エントリーDOI: 10.2210/pdb2iny/pdb
• 関連するPDBエントリー: 1HX6 1M3Y 1P2Z 1P30 2BBD
• 分子名称: Hexon protein (1 entity in total)
• 機能のキーワード: avian adenovirus, celo, major coat protein, hexon, crystal packing, nanotechnology, viral jelly roll, viral protein
• 由来する生物種: Fowl adenovirus 1
• 細胞内の位置: Virion : P42671
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 106795.63

構造登録者

Xu, L.,Benson, S.D.,Burnett, R.M. (登録日: 2006-10-09, 公開日: 2007-02-06, 最終更新日: 2023-08-30)

主引用文献

Xu, L.,Benson, S.D.,Burnett, R.M.
Nanoporous crystals of chicken embryo lethal orphan (CELO) adenovirus major coat protein, hexon.
J.Struct.Biol., 157:424-431, 2007

PubMed Abstract: CELO (chicken embryo lethal orphan) virus is an avian adenovirus that is being developed as a gene transfer vector. Its trimeric major coat protein (942 residues, 106,709 Da) has 42% sequence identity to human adenovirus type 2 (AdH2) hexon and 45% to AdH5 hexon. For structural studies, the growth of CELO virus has been optimized, and its hexon purified and crystallized. The hexon crystals, the first non-human example, diffract to 3.9 A resolution. Molecular replacement using the AdH5 model was used to identify the location of the CELO hexon within the unit cell. There is one hexon monomer in the asymmetric unit of the trigonal space group P321 (a=b=157.8 A, c=114.2 A, gamma=120 degrees) and the solvent content is 67.8%. The hexons pack in a hexagonal honeycomb so that large approximately 100 A diameter channels run through the entire crystal. This remarkable property of the crystals lends itself to their exploitation as a nanomaterial. Structural studies on CELO will elucidate the differences between avian and human adenoviruses and contribute to a better understanding of adenoviruses with non-human hosts.

PubMed: 17071105
DOI: 10.1016/j.jsb.2006.08.017

実験手法

X-RAY DIFFRACTION (3.9 Å)