2ILK

CRYSTAL STRUCTURE OF HUMAN INTERLEUKIN-10 AT 1.6 ANGSTROMS RESOLUTION

2ILK の概要

• エントリーDOI: 10.2210/pdb2ilk/pdb
• 分子名称: INTERLEUKIN-10, SULFATE ION (3 entities in total)
• 機能のキーワード: cytokine
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P22301
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18960.64

構造登録者

Zdanov, A.,Schalk-Hihi, C.,Wlodawer, A. (登録日: 1996-07-01, 公開日: 1996-10-14, 最終更新日: 2024-11-13)

主引用文献

Zdanov, A.,Schalk-Hihi, C.,Wlodawer, A.
Crystal structure of human interleukin-10 at 1.6 A resolution and a model of a complex with its soluble receptor.
Protein Sci., 5:1955-1962, 1996

PubMed Abstract: The crystal structure of human interleukin-10 (IL-10) was refined at 1.6 A resolution against X-ray diffraction data collected at 100 K with the use of synchrotron radiation. Although similar to the IL-10 structure determined previously at room temperature, this low-temperature IL-10 structure contains, in addition, four N-terminal residues, three sulfate anions, and 175 extra water molecules. Whereas the main-chain conformation is preserved, about 30% of the side chains, most of them on the protein surface, assume different conformations. A computer model of a complex of IL-10 with its two soluble receptors was generated based on the topological similarity of IL-10 to interferon-gamma. The contact region between the cytokine and each receptor shows excellent complementarity of polar and hydrophobic interactions, suggesting that the model is generally correct and should be useful in guiding mutagenesis experiments.

PubMed: 8897595

実験手法

X-RAY DIFFRACTION (1.6 Å)