2IH1

Ion selectivity in a semi-synthetic K+ channel locked in the conductive conformation

2IH1 の概要

• エントリーDOI: 10.2210/pdb2ih1/pdb
• 関連するPDBエントリー: 2IH3
• 分子名称: FAB Heavy Chain, FAB Light Chain, Voltage-gated potassium channel, ... (6 entities in total)
• 機能のキーワード: ion channel d-amino acid semi-synthetic, membrane protein
• 由来する生物種: Streptomyces lividans; 詳細
• 細胞内の位置: Cell membrane; Multi-pass membrane protein: P0A334
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 60513.58

構造登録者

Valiyaveetil, F.I.,Leonetti, M.,Muir, T.W.,MacKinnon, R. (登録日: 2006-09-25, 公開日: 2006-11-21, 最終更新日: 2024-10-09)

主引用文献

Valiyaveetil, F.I.,Leonetti, M.,Muir, T.W.,Mackinnon, R.
Ion Selectivity in a Semisynthetic K+ Channel Locked in the Conductive Conformation.
Science, 314:1004-1007, 2006

PubMed Abstract: Potassium channels are K+-selective protein pores in cell membrane. The selectivity filter is the functional unit that allows K+ channels to distinguish potassium (K+) and sodium (Na+) ions. The filter's structure depends on whether K+ or Na+ ions are bound inside it. We synthesized a K+ channel containing the d-enantiomer of alanine in place of a conserved glycine and found by x-ray crystallography that its filter maintains the K+ (conductive) structure in the presence of Na+ and very low concentrations of K+. This channel conducts Na+ in the absence of K+ but not in the presence of K+. These findings demonstrate that the ability of the channel to adapt its structure differently to K+ and Na+ is a fundamental aspect of ion selectivity, as is the ability of multiple K+ ions to compete effectively with Na+ for the conductive filter.

PubMed: 17095703
DOI: 10.1126/science.1133415

実験手法

X-RAY DIFFRACTION (2.4 Å)