2IFF

STRUCTURE OF AN ANTIBODY-LYSOZYME COMPLEX: EFFECT OF A CONSERVATIVE MUTATION

Summary for 2IFF

• Entry DOI: 10.2210/pdb2iff/pdb
• Descriptor: IGG1 HYHEL-5 FAB (LIGHT CHAIN), IGG1 HYHEL-5 FAB (HEAVY CHAIN), HEN EGG WHITE LYSOZYME, ... (4 entities in total)
• Functional Keywords: immunoglobulin/hydrolase(o-glycosyl), immunoglobulin-hydrolase(o-glycosyl) complex
• Biological source: Mus musculus (house mouse); More
• Cellular location: Secreted: P00698
• Total number of polymer chains: 3
• Total formula weight: 60605.32

Authors

Chacko, S.,Davies, D.R. (deposition date: 1994-02-03, release date: 1994-05-31, Last modification date: 2024-11-13)

Primary citation

Chacko, S.,Silverton, E.,Kam-Morgan, L.,Smith-Gill, S.,Cohen, G.,Davies, D.
Structure of an antibody-lysozyme complex unexpected effect of conservative mutation.
J.Mol.Biol., 245:261-274, 1995

PubMed Abstract: The structure of the complex between the Fab HyHEL-5 and chicken lysozyme revealed a large interface region containing 23 lysozyme and 28 Fab residues. Arg68 of the lysozyme is centrally placed in this interface and theoretical studies together with binding assays of this Fab to different avian lysozymes have previously shown that this arginine residue is an important contributor to the binding. The Arg68-->Lys mutant binds 10(3) times less well to the HyHEL-5 Fab. We have examined the refined crystal structure of the complex of this mutant lysozyme with the Fab. No global changes occur, but there is an introduction of a new water molecule into the interface that mediates the hydrogen bonding interactions between the lysine and residues on the Fab. These data are compared with the effects of similar changes on the inhibition of serine proteases such as trypsin where the energetic effects of this substitution are small.

PubMed: 7531245
DOI: 10.1006/jmbi.1994.0022

Experimental method

X-RAY DIFFRACTION (2.65 Å)