2IDS

Structure of M98A mutant of amicyanin, Cu(I)

2IDS の概要

• エントリーDOI: 10.2210/pdb2ids/pdb
• 関連するPDBエントリー: 2IDQ 2IDT 2IDU
• 分子名称: Amicyanin, COPPER (I) ION (3 entities in total)
• 機能のキーワード: blue copper protein; beta sandwich, electron transport
• 由来する生物種: Paracoccus denitrificans
• 細胞内の位置: Periplasm: P22364
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11508.60

構造登録者

Carrell, C.J.,Ma, J.K.,Antholine, W.,Hosler, J.P.,Mathews, F.S.,Davidson, V.L. (登録日: 2006-09-15, 公開日: 2007-03-13, 最終更新日: 2023-08-30)

主引用文献

Carrell, C.J.,Ma, J.K.,Antholine, W.E.,Hosler, J.P.,Mathews, F.S.,Davidson, V.L.
Generation of Novel Copper Sites by Mutation of the Axial Ligand of Amicyanin. Atomic Resolution Structures and Spectroscopic Properties
Biochemistry, 46:1900-1912, 2007

PubMed Abstract: Amicyanin from Paracoccus denitrificans is a type 1 copper protein with three strong equatorial copper ligands provided by nitrogens of His53 and His95 and the sulfur of Cys92, with an additional weak axial ligand provided by the sulfur of Met98. Met98 was replaced with either Gln or Ala. As isolated, the M98A and M98Q mutant proteins contain zinc in the active site. The zinc is then removed and replaced with copper so that the copper-containing proteins may be studied. Each of the mutant amicyanins exhibits a marked decrease in thermal stability relative to that of native amicyanin, consistent with the weaker affinity for copper. Crystal structures were obtained for the oxidized and reduced forms of M98A and M98Q amicyanins at atomic resolution (PubMed: 17295442
DOI: 10.1021/bi0619674

実験手法

X-RAY DIFFRACTION (1 Å)