2IC2

Crystal Structure of the First FNIII Domain of Ihog

2IC2 の概要

• エントリーDOI: 10.2210/pdb2ic2/pdb
• 関連するPDBエントリー: 2IBB 2IBG
• 分子名称: CG9211-PA, SULFATE ION (3 entities in total)
• 機能のキーワード: ihog, hedgehog, fibronectin type iii, protein binding
• 由来する生物種: Drosophila melanogaster (fruit fly)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27321.66

構造登録者

McLellan, J.S.,Leahy, D.J. (登録日: 2006-09-12, 公開日: 2006-10-24, 最終更新日: 2024-11-20)

主引用文献

McLellan, J.S.,Yao, S.,Zheng, X.,Geisbrecht, B.V.,Ghirlando, R.,Beachy, P.A.,Leahy, D.J.
Structure of a heparin-dependent complex of Hedgehog and Ihog.
Proc.Natl.Acad.Sci.Usa, 103:17208-17213, 2006

PubMed Abstract: Hedgehog (Hh) signaling molecules mediate key tissue-patterning events during animal development, and inappropriate activation of Hh signaling in adults has been associated with human cancers. Recently, a conserved family of type I integral membrane proteins required for normal response to the Hh signal was discovered. One member of this family, Ihog (interference hedgehog), functions upstream or at the level of Patched (Ptc), but how Ihog participates in Hh signaling remains unclear. Here, we show that heparin binding induces Ihog dimerization and is required to mediate high-affinity interactions between Ihog and Hh. We also present crystal structures of a Hh-binding fragment of Ihog, both alone and complexed with Hh. Heparin is not well ordered in these structures, but a basic cleft in the first FNIII domain of Ihog (IhogFn1) is shown by mutagenesis to mediate heparin binding. These results establish that Hh directly binds Ihog and provide the first demonstration of a specific role for heparin in Hh responsiveness.

PubMed: 17077139
DOI: 10.1073/pnas.0606738103

実験手法

X-RAY DIFFRACTION (1.3 Å)