2IB9

Crystallographic and kinetic studies of human mitochondrial acetoacetyl-CoA thiolase (T2): the importance of potassium and chloride for its structure and function

2IB9 の概要

• エントリーDOI: 10.2210/pdb2ib9/pdb
• 関連するPDBエントリー: 2IB7 2IB8 2IBU 2IBW 2IBY
• 分子名称: Acetyl-CoA acetyltransferase, CHLORIDE ION, POTASSIUM ION, ... (6 entities in total)
• 機能のキーワード: thiolase fold, potassium ion, chloride, beta-alpha-beta-alpha-beta-alpha-beta-beta topology, alpha-beta-alpha-beta-alpha layered structure, transferase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Mitochondrion: P24752
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 167671.81

構造登録者

Haapalainen, A.M.,Wierenga, R.K. (登録日: 2006-09-11, 公開日: 2007-04-03, 最終更新日: 2023-10-25)

主引用文献

Haapalainen, A.M.,Merilainen, G.,Pirila, P.L.,Kondo, N.,Fukao, T.,Wierenga, R.K.
Crystallographic and Kinetic Studies of Human Mitochondrial Acetoacetyl-CoA Thiolase: The Importance of Potassium and Chloride Ions for Its Structure and Function
Biochemistry, 46:4305-4321, 2007

PubMed Abstract: Thiolases are CoA-dependent enzymes which catalyze the formation of a carbon-carbon bond in a Claisen condensation step and its reverse reaction via a thiolytic degradation mechanism. Mitochondrial acetoacetyl-coenzyme A (CoA) thiolase (T2) is important in the pathways for the synthesis and degradation of ketone bodies as well as for the degradation of 2-methylacetoacetyl-CoA. Human T2 deficiency has been identified in more than 60 patients. A unique property of T2 is its activation by potassium ions. High-resolution human T2 crystal structures are reported for the apo form and the CoA complex, with and without a bound potassium ion. The potassium ion is bound near the CoA binding site and the catalytic site. Binding of the potassium ion at this low-affinity binding site causes the rigidification of a CoA binding loop and an active site loop. Unexpectedly, a high-affinity binding site for a chloride ion has also been identified. The chloride ion is copurified, and its binding site is at the dimer interface, near two catalytic loops. A unique property of T2 is its ability to use 2-methyl-branched acetoacetyl-CoA as a substrate, whereas the other structurally characterized thiolases cannot utilize the 2-methylated compounds. The kinetic measurements show that T2 can degrade acetoacetyl-CoA and 2-methylacetoacetyl-CoA with similar catalytic efficiencies. For both substrates, the turnover numbers increase approximately 3-fold when the potassium ion concentration is increased from 0 to 40 mM KCl. The structural analysis of the active site of T2 indicates that the Phe325-Pro326 dipeptide near the catalytic cavity is responsible for the exclusive 2-methyl-branched substrate specificity.

PubMed: 17371050
DOI: 10.1021/bi6026192

実験手法

X-RAY DIFFRACTION (2.05 Å)