2I9E

Structure of Triosephosphate Isomerase of Tenebrio molitor

2I9E の概要

• エントリーDOI: 10.2210/pdb2i9e/pdb
• 分子名称: Triosephosphate isomerase, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL (3 entities in total)
• 機能のキーワード: triosephosphate isomerase tenebrio molitor, isomerase
• 由来する生物種: Tenebrio molitor (yellow mealworm)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 113379.62

構造登録者

Schmidt, A.,Scheerer, P.,Wessner, H.,Hoehne, W.,Krauss, N. (登録日: 2006-09-05, 公開日: 2006-09-26, 最終更新日: 2023-08-30)

主引用文献

Knobeloch, D.,Schmidt, A.,Scheerer, P.,Krauss, N.,Wessner, H.,Scholz, C.,Kuttner, G.,von Rintelen, T.,Wessel, A.,Hohne, W.
A coleopteran triosephosphate isomerase: X-ray structure and phylogenetic impact of insect sequences.
Insect Mol Biol, 19:35-48, 2010

PubMed Abstract: A coleopteran triosephosphate isomerase (TIM) from Tenebrio molitor (yellow mealworm beetle) was recombinantly expressed in Escherichia coli and characterized with respect to thermal stability, kinetic parameters and oligomeric state. The enzyme was successfully crystallized and the structure determined by X-ray analysis to 2.0 A resolution. This is the first example of an invertebrate TIM. We compare structural features with known structures of TIMs from microorganisms, plants and vertebrates, and discuss the utility of the Tenebrio TIM sequence, together with several newly sequenced insect TIMs, for molecular phylogenetic analysis.

PubMed: 19849721
DOI: 10.1111/j.1365-2583.2009.00928.x

実験手法

X-RAY DIFFRACTION (2 Å)