2I3B

Solution Structure of a Human Cancer-Related Nucleoside Triphosphatase

2I3B の概要

• エントリーDOI: 10.2210/pdb2i3b/pdb
• NMR情報: BMRB: 7119
• 分子名称: Human Cancer-Related NTPase (1 entity in total)
• 機能のキーワード: ntpase, aaa, rossmann, hydrolase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20610.87

構造登録者

Placzek, W.J.,Almeida, M.S.,Wuthrich, K. (登録日: 2006-08-17, 公開日: 2007-03-13, 最終更新日: 2024-05-29)

主引用文献

Placzek, W.J.,Almeida, M.S.,Wuthrich, K.
NMR Structure and Functional Characterization of a Human Cancer-related Nucleoside Triphosphatase.
J.Mol.Biol., 367:788-801, 2007

PubMed Abstract: A screen of the human cancer genome anatomy project (CGAP) database was performed to search for new proteins involved in tumorigenesis. The resulting hits were further screened for recombinant expression, solubility and protein aggregation, which led to the identification of the previously unknown human cancer-related (HCR) protein encoded by the mRNA NM_032324 as a target for structure determination by NMR. The three-dimensional structure of the protein in its complex with ATPgammaS forms a three-layered alpha/beta sandwich, with a central nine-stranded beta-sheet surrounded by five alpha-helices. Sequence and three-dimensional structure comparisons with AAA+ ATPases revealed the presence of Walker A (GPPGVGKT) and Walker B (VCVIDEIG) motifs. Using 1D (31)P-NMR spectroscopy and a coupled enzymatic assay for the determination of inorganic phosphate, we showed that the purified recombinant protein is active as a non-specific nucleoside triphosphatase, with k(cat)=7.6x10(-3) s(-1). The structural basis for the enzymatic activity of HCR-NTPase was further characterized by site-directed mutagenesis of the Walker B motif, which further contributes to making the HCR-NTPase an attractive new target for further biochemical characterization in the context of its presumed role in human tumorigenesis.

PubMed: 17291528
DOI: 10.1016/j.jmb.2007.01.001

実験手法

SOLUTION NMR