2I37

Crystal structure of a photoactivated rhodopsin

2I37 の概要

• エントリーDOI: 10.2210/pdb2i37/pdb
• 関連するPDBエントリー: 2I35 2I36
• 分子名称: Rhodopsin, alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: trans-membrane protein, gpcr, membrane protein
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Membrane; Multi-pass membrane protein: P02699
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 120732.78

構造登録者

Lodowski, D.T.,Stenkamp, R.E.,Salom, D.,Le Trong, I.,Palczewski, K. (登録日: 2006-08-17, 公開日: 2006-10-17, 最終更新日: 2024-10-30)

主引用文献

Salom, D.,Lodowski, D.T.,Stenkamp, R.E.,Trong, I.L.,Golczak, M.,Jastrzebska, B.,Harris, T.,Ballesteros, J.A.,Palczewski, K.
Crystal structure of a photoactivated deprotonated intermediate of rhodopsin.
Proc.Natl.Acad.Sci.Usa, 103:16123-16128, 2006

PubMed Abstract: The changes that lead to activation of G protein-coupled receptors have not been elucidated at the structural level. In this work we report the crystal structures of both ground state and a photoactivated deprotonated intermediate of bovine rhodopsin at a resolution of 4.15 A. In the photoactivated state, the Schiff base linking the chromophore and Lys-296 becomes deprotonated, reminiscent of the G protein-activating state, metarhodopsin II. The structures reveal that the changes that accompany photoactivation are smaller than previously predicted for the metarhodopsin II state and include changes on the cytoplasmic surface of rhodopsin that possibly enable the coupling to its cognate G protein, transducin. Furthermore, rhodopsin forms a potentially physiologically relevant dimer interface that involves helices I, II, and 8, and when taken with the prior work that implicates helices IV and V as the physiological dimer interface may account for one of the interfaces of the oligomeric structure of rhodopsin seen in the membrane by atomic force microscopy. The activation and oligomerization models likely extend to the majority of other G protein-coupled receptors.

PubMed: 17060607
DOI: 10.1073/pnas.0608022103

実験手法

X-RAY DIFFRACTION (4.15 Å)