2I1Q

RadA Recombinase in complex with Calcium

2I1Q の概要

• エントリーDOI: 10.2210/pdb2i1q/pdb
• 関連するPDBエントリー: 1Z4D
• 分子名称: DNA repair and recombination protein radA, MAGNESIUM ION, CALCIUM ION, ... (6 entities in total)
• 機能のキーワード: atpase, recombinase, atp complex, calcium stimulation, reca, rad51, dmc1, rada, recombination
• 由来する生物種: Methanococcus voltae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35872.65

構造登録者

Qian, X.,He, Y.,Ma, X.,Fodje, M.N.,Grochulski, P.,Luo, Y. (登録日: 2006-08-14, 公開日: 2006-10-24, 最終更新日: 2023-08-30)

主引用文献

Qian, X.,He, Y.,Ma, X.,Fodje, M.N.,Grochulski, P.,Luo, Y.
Calcium Stiffens Archaeal Rad51 Recombinase from Methanococcus voltae for Homologous Recombination.
J.Biol.Chem., 281:39380-39387, 2006

PubMed Abstract: Archaeal RadA or Rad51 recombinases are close homologues of eukaryal Rad51 and DMC1. These and bacterial RecA orthologues play a key role in DNA repair by forming helical nucleoprotein filaments in which a hallmark strand exchange reaction between homologous DNA substrates occurs. Recent studies have discovered the stimulatory role by calcium on human and yeast recombinases. Here we report that the strand exchange activity but not the ATPase activity of an archaeal RadA/Rad51 recombinase from Methanococcus voltae (MvRadA) is also subject to calcium stimulation. Crystallized MvRadA filaments in the presence of CaCl(2) resemble that of the recently reported ATPase active form in the presence of an activating dose of KCl. At the ATPase center, one Ca(2+) ion takes the place of two K(+) ions in the K(+)-bound form. The terminal phosphate of the nonhydrolyzable ATP analogue is in a staggered conformation in the Ca(2+)-bound form. In comparison, an eclipsed conformation was seen in the K(+)-bound form. Despite the changes in the ATPase center, both forms harbor largely ordered L2 regions in essentially identical conformations. These data suggest a unified stimulation mechanism by potassium and calcium because of the existence of a conserved ATPase center promiscuous in binding cations.

PubMed: 17050545
DOI: 10.1074/jbc.M607785200

実験手法

X-RAY DIFFRACTION (1.9 Å)