2HYW

Human Annexin A2 with Calcium bound

2HYW の概要

• エントリーDOI: 10.2210/pdb2hyw/pdb
• 分子名称: Annexin A2, CALCIUM ION (3 entities in total)
• 機能のキーワード: calcium-binding protein, membrane-binding protein, helix bundle, metal binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted, extracellular space, extracellular matrix, basement membrane : P07355
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 71265.78

構造登録者

Shao, C.,Head, J.F.,Seaton, B.A. (登録日: 2006-08-07, 公開日: 2006-09-05, 最終更新日: 2023-08-30)

主引用文献

Shao, C.,Zhang, F.,Kemp, M.M.,Linhardt, R.J.,Waisman, D.M.,Head, J.F.,Seaton, B.A.
Crystallographic Analysis of Calcium-dependent Heparin Binding to Annexin A2.
J.Biol.Chem., 281:31689-31695, 2006

PubMed Abstract: Annexin A2 and heparin bind to one another with high affinity and in a calcium-dependent manner, an interaction that may play a role in mediating fibrinolysis. In this study, three heparin-derived oligosaccharides of different lengths were co-crystallized with annexin A2 to elucidate the structural basis of the interaction. Crystal structures were obtained at high resolution for uncomplexed annexin A2 and three complexes of heparin oligosaccharides bound to annexin A2. The common heparin-binding site is situated at the convex face of domain IV of annexin A2. At this site, annexin A2 binds up to five sugar residues from the nonreducing end of the oligosaccharide. Unlike most heparin-binding consensus patterns, heparin binding at this site does not rely on arrays of basic residues; instead, main-chain and side-chain nitrogen atoms and two calcium ions play important roles in the binding. Especially significant is a novel calcium-binding site that forms upon heparin binding. Two sugar residues of the heparin derivatives provide oxygen ligands for this calcium ion. Comparison of all four structures shows that heparin binding does not elicit a significant conformational change in annexin A2. Finally, surface plasmon resonance measurements were made for binding interactions between annexin A2 and heparin polysaccharide in solution at pH 7.4 or 5.0. The combined data provide a clear basis for the calcium dependence of heparin binding to annexin A2.

PubMed: 16882661
DOI: 10.1074/jbc.M604502200

実験手法

X-RAY DIFFRACTION (2.1 Å)