2HVD

Human nucleoside diphosphate kinase A complexed with ADP

2HVD の概要

• エントリーDOI: 10.2210/pdb2hvd/pdb
• 関連するPDBエントリー: 1JXV 1UCN 2HVE
• 分子名称: Nucleoside diphosphate kinase A, ADENOSINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: complex adp, signaling protein, transferase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm : P15531
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 52793.77

構造登録者

Giraud, M.-F.,Georgescauld, F.,Lascu, I.,Dautant, A. (登録日: 2006-07-28, 公開日: 2006-09-19, 最終更新日: 2024-10-16)

主引用文献

Giraud, M.-F.,Georgescauld, F.,Lascu, I.,Dautant, A.
Crystal Structures of S120G Mutant and Wild Type of Human Nucleoside Diphosphate Kinase A in Complex with ADP
J.Bioenerg.Biomembr., 38:261-264, 2006

PubMed Abstract: Nm23 was the first metastasis suppressor gene identified. This gene encodes a NDP kinase that also exhibits other properties like histidine protein kinase and interactions with proteins and DNA. The S120G mutant of NDPK-A has been identified in aggressive neuroblastomas and has been found to reduce the metastasis suppressor effect of Nm23. In order to understand the differences between the wild type and the S120G mutant, we have determined the structure of both mutant and wild type NDPK-A in complex with ADP. Our results reveal that there are no significant changes between the two enzyme versions even in the surroundings of the catalytic histidine that is required for NDP kinase activity. This suggests that the S120G mutation may affect an other protein property than NDP kinase activity.

PubMed: 16944299
DOI: 10.1007/s10863-006-9043-0

実験手法

X-RAY DIFFRACTION (2.15 Å)