2HPS

Crystal structure of coelenterazine-binding protein from Renilla Muelleri

2HPS の概要

• エントリーDOI: 10.2210/pdb2hps/pdb
• 分子名称: coelenterazine-binding protein with bound coelenterazine, C2-HYDROXY-COELENTERAZINE, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: bioluminescence, coelenterazine-binding, southeast collaboratory for structural genomics, secsg, structural genomics, psi, protein structure initiative, luminescent protein
• 由来する生物種: Renilla muelleri
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21757.68

構造登録者

Stepanyuk, G.,Liu, Z.J.,Vysotski, E.S.,Lee, J.,Rose, J.P.,Wang, B.C.,Southeast Collaboratory for Structural Genomics (SECSG) (登録日: 2006-07-17, 公開日: 2007-01-09, 最終更新日: 2024-10-30)

主引用文献

Stepanyuk, G.A.,Liu, Z.J.,Markova, S.S.,Frank, L.A.,Lee, J.,Vysotski, E.S.,Wang, B.C.
Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: why it is not a calcium-regulated photoprotein.
PHOTOCHEM.PHOTOBIOL.SCI., 7:442-447, 2008

PubMed Abstract: Bioluminescence in the sea pansy Renilla involves two distinct proteins, a Ca2+-triggered coelenterazine-binding protein (CBP), and Renilla luciferase. CBP contains one tightly bound coelenterazine molecule, which becomes available for reaction with luciferase and O2 only subsequent to Ca2+ binding. CBP belongs to the EF-hand superfamily of Ca2+-binding proteins and contains three "EF-hand" Ca2+-binding sites. The overall spatial structure of recombinant selenomethionine-labeled CBP determined at 1.7 A, is found to approximate the protein scaffold characteristic of the class of Ca2+-regulated photoproteins. Photoproteins however, catalyze molecular oxygen addition to coelenterazine producing a 2-hydroperoxycoelenterazine intermediate, which is stabilized within the binding cavity in the absence of Ca2+. Addition of Ca2+ triggers the bioluminescence reaction. However in CBP this first step of oxygen addition is not allowed. The different amino acid environments and hydrogen bond interactions within the binding cavity, are proposed to account for the different properties of the two classes of proteins.

PubMed: 18385886
DOI: 10.1039/b716535h

実験手法

X-RAY DIFFRACTION (1.72 Å)