2HPM

Eubacterial and Eukaryotic Replicative DNA Polymerases are not Homologous: X-ray Structure of DNA Polymerase III

2HPM の概要

• エントリーDOI: 10.2210/pdb2hpm/pdb
• 関連するPDBエントリー: 2HPI
• 分子名称: DNA Polymerase III alpha subunit, ZINC ION, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: nucleotidyltransferase fold, transferase
• 由来する生物種: Thermus aquaticus
• 細胞内の位置: Cytoplasm (By similarity): Q9XDH5
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 138087.71

構造登録者

Bailey, S.,Wing, R.A.,Steitz, T.A. (登録日: 2006-07-17, 公開日: 2006-09-19, 最終更新日: 2024-02-14)

主引用文献

Bailey, S.,Wing, R.A.,Steitz, T.A.
The Structure of T. aquaticus DNA Polymerase III Is Distinct from Eukaryotic Replicative DNA Polymerases.
Cell(Cambridge,Mass.), 126:893-904, 2006

PubMed Abstract: The crystal structure of Thermus aquaticus DNA polymerase III alpha subunit reveals that the structure of the catalytic domain of the eubacterial replicative polymerase is unrelated to that of the eukaryotic replicative polymerase but rather belongs to the Polbeta-like nucleotidyltransferase superfamily. A model of the polymerase complexed with both DNA and beta-sliding clamp interacting with a reoriented binding domain and internal beta binding site was constructed that is consistent with existing biochemical data. Within the crystal, two C-terminal domains are interacting through a surface that is larger than many dimer interfaces. Since replicative polymerases of eubacteria and eukaryotes/archaea are not homologous, the nature of the replicative polymerase in the last common ancestor is unknown. Although other possibilities have been proposed, the plausibility of a ribozyme DNA polymerase should be considered.

PubMed: 16959569
DOI: 10.1016/j.cell.2006.07.027

実験手法

X-RAY DIFFRACTION (3.7 Å)