2HP0

Crystal structure of iminodisuccinate epimerase

2HP0 の概要

• エントリーDOI: 10.2210/pdb2hp0/pdb
• 分子名称: IDS-epimerase, 1,2-ETHANEDIOL, UNKNOWN ATOM OR ION, ... (6 entities in total)
• 機能のキーワード: mmge/prpd fold, 6 helix bundle, chorismate mutase like, isomerase
• 由来する生物種: Agrobacterium tumefaciens; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 101874.23

構造登録者

Lohkamp, B.,Bauerle, B.,Rieger, P.G.,Schneider, G. (登録日: 2006-07-17, 公開日: 2006-09-12, 最終更新日: 2024-11-13)

主引用文献

Lohkamp, B.,Bauerle, B.,Rieger, P.G.,Schneider, G.
Three-dimensional Structure of Iminodisuccinate Epimerase Defines the Fold of the MmgE/PrpD Protein Family.
J.Mol.Biol., 362:555-566, 2006

PubMed Abstract: Iminodisuccinate (IDS) epimerase catalyzes the epimerisation of R,R-, S,S- and R,S- iminodisuccinate, one step in the biodegradation of the chelating agent iminodisuccinate by Agrobacterium tumefaciens BY6. The enzyme is a member of the MmgE/PrpD protein family, a diverse and little characterized class of proteins of prokaryotic and eukaryotic origin. IDS epimerase does not show significant overall amino acid sequence similarity to any other protein of known three-dimensional structure. The crystal structure of this novel epimerase has been determined by multi-wavelength diffraction to 1.5 A resolution using selenomethionine-substituted enzyme. In the crystal, the enzyme forms a homo-dimer, and the subunit consists of two domains. The larger domain, not consecutive in sequence and comprising residues Met1-Lys266 and Leu400-Pro446, forms a novel all alpha-helical fold with a central six-helical bundle. The second, smaller domain folds into an alpha+beta domain, related in topology to chorismate mutase by a circular permutation. IDS epimerase is thus not related in three-dimensional structure to other known epimerases. The fold of the IDS epimerase is representative for the whole MmgE/PrpD family. The putative active site is located at the interface between the two domains of the subunit, and is characterized by a positively charged surface, consistent with the binding of a highly negatively charged substrate such as iminodisuccinate. Docking experiments suggest a two-base mechanism for the epimerisation reaction.

PubMed: 16934291
DOI: 10.1016/j.jmb.2006.07.051

実験手法

X-RAY DIFFRACTION (1.5 Å)