2HN2

Crystal structure of the CorA Mg2+ transporter homologue from T. maritima in complex with divalent cations

2HN2 の概要

• エントリーDOI: 10.2210/pdb2hn2/pdb
• 関連するPDBエントリー: 2BBH 2BBJ 2HN1 2IUB
• 分子名称: Magnesium transport protein corA, CALCIUM ION (2 entities in total)
• 機能のキーワード: integral membrane protein; metal transporter protein; divalent cations, metal transport
• 由来する生物種: Thermotoga maritima
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 209422.79

構造登録者

Payandeh, J.,Pai, E.F. (登録日: 2006-07-11, 公開日: 2006-08-29, 最終更新日: 2023-08-30)

主引用文献

Payandeh, J.,Pai, E.F.
A structural basis for Mg(2+) homeostasis and the CorA translocation cycle.
Embo J., 25:3762-3773, 2006

PubMed Abstract: We describe the CorA Mg(2+) transporter homologue from Thermotoga maritima in complex with 12 divalent cations at 3.7 A resolution. One metal is found near the universally conserved GMN motif, apparently stabilized within the transmembrane region. This portion of the selectivity filter might discriminate between the size and preferred coordination geometry of hydrated substrates. CorA may further achieve specificity by requiring the sequential dehydration of substrates along the length of its approximately 55 A long pore. Ten metal sites identified within the cytoplasmic funnel domain are linked to long extensions of the pore helices and regulate the transport status of CorA. We have characterized this region as an intrinsic divalent cation sensor and provide evidence that it functions as a Mg(2+)-specific homeostatic molecular switch. A proteolytic protection assay, biophysical data, and comparison to a soluble domain structure from Archaeoglobus fulgidus have revealed the potential reaction coordinate for this diverse family of transport proteins.

PubMed: 16902408
DOI: 10.1038/sj.emboj.7601269

実験手法

X-RAY DIFFRACTION (3.7 Å)