2HKY

NMR solution structure of human RNase 7

2HKY の概要

• エントリーDOI: 10.2210/pdb2hky/pdb
• 分子名称: Ribonuclease 7 (1 entity in total)
• 機能のキーワード: rnase, antimicrobial activity, hydrolase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: Q9H1E1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14714.19

構造登録者

Huang, Y.-C.,Chen, C.,Lou, Y.-C. (登録日: 2006-07-06, 公開日: 2006-12-26, 最終更新日: 2024-10-30)

主引用文献

Huang, Y.C.,Lin, Y.M.,Chang, T.W.,Wu, S.J.,Lee, Y.S.,Chang, M.D.,Chen, C.,Wu, S.H.,Liao, Y.D.
The flexible and clustered lysine residues of human ribonuclease 7 are critical for membrane permeability and antimicrobial activity.
J.Biol.Chem., 282:4626-4633, 2007

PubMed Abstract: The ubiquitous ribonucleases (RNases) play important roles in RNA metabolism, angiogenesis, neurotoxicity, and antitumor or antimicrobial activity. Only the antimicrobial RNases possess high positively charged residues, although their mechanisms of action remain unclear. Here, we report on the role of cationic residues of human RNase7 (hRNase7) in its antimicrobial activity. It exerted antimicrobial activity against bacteria and yeast, even at 4 degrees C. The bacterial membrane became permeable to the DNA-binding dye SYTOX(R) Green in only a few minutes after bactericidal RNase treatment. NMR studies showed that the 22 positively charged residues (Lys(18) and Arg(4)) are distributed into three clusters on the surface of hRNase7. The first cluster, K(1),K(3),K(111),K(112), was located at the flexible coil near the N terminus, whereas the other two, K(32),K(35) and K(96),R(97),K(100), were located on rigid secondary structures. Mutagenesis studies showed that the flexible cluster K(1),K(3),K(111),K(112), rather than the catalytic residues His(15), Lys(38), and His(123) or other clusters such as K(32),K(35) and K(96),R(97),K(100), is critical for the bactericidal activity. We suggest that the hRNase7 binds to bacterial membrane and renders the membrane permeable through the flexible and clustered Lys residues K(1),K(3),K(111),K(112). The conformation of hRNase7 can be adapted for pore formation or disruption of bacterial membrane even at 4 degrees C.

PubMed: 17150966
DOI: 10.1074/jbc.M607321200

実験手法

SOLUTION NMR