2HHK

Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylglycerol

Summary for 2HHK

• Entry DOI: 10.2210/pdb2hhk/pdb
• Related: 2HG3 2HG9 2HH1 2HIT 2HJ6
• Descriptor: Reaction center protein L chain, PHOSPHATE ION, CARDIOLIPIN, ... (16 entities in total)
• Functional Keywords: photosynthesis, photosynthetic reaction center, lipid binding sites, brominated lipid, membrane protein, photosynthesis-membrane protein complex, photosynthesis/membrane protein
• Biological source: Rhodobacter sphaeroides; More
• Cellular location: Cellular chromatophore membrane; Multi-pass membrane protein: P0C0Y8 P0C0Y9; Cellular chromatophore membrane; Single-pass membrane protein: P0C0Y7
• Total number of polymer chains: 3
• Total formula weight: 106341.33

Authors

Roszak, A.W.,Gardiner, A.T.,Isaacs, N.W.,Cogdell, R.J. (deposition date: 2006-06-28, release date: 2007-03-27, Last modification date: 2024-04-03)

Primary citation

Roszak, A.W.,Gardiner, A.T.,Isaacs, N.W.,Cogdell, R.J.
Brominated Lipids Identify Lipid Binding Sites on the Surface of the Reaction Center from Rhodobacter sphaeroides.
Biochemistry, 46:2909-2916, 2007

PubMed Abstract: This study describes the use of brominated phospholipids to distinguish between lipid and detergent binding sites on the surface of a typical alpha-helical membrane protein. Reaction centers isolated from Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structural analysis of these crystals has revealed the presence of two lipid binding sites from the characteristic strong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approach to mapping lipid binding sites at the surface of membrane proteins.

PubMed: 17315985
DOI: 10.1021/bi062154i

Experimental method

X-RAY DIFFRACTION (2.5 Å)