2HGH

Transcription Factor IIIA zinc fingers 4-6 bound to 5S rRNA 55mer (NMR structure)

2HGH の概要

• エントリーDOI: 10.2210/pdb2hgh/pdb
• 関連するPDBエントリー: 1TF3 1ZNF
• NMR情報: BMRB: 7194
• 分子名称: 55-MER, Transcription factor IIIA, ZINC ION (3 entities in total)
• 機能のキーワード: zinc finger, transcription-rna complex, transcription/rna
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• 細胞内の位置: Nucleus: P03001
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28122.48

構造登録者

Lee, B.M. (登録日: 2006-06-27, 公開日: 2006-08-01, 最終更新日: 2024-05-29)

主引用文献

Lee, B.M.,Xu, J.,Clarkson, B.K.,Martinez-Yamout, M.A.,Dyson, J.H.,Case, D.A.,Gottesfeld, J.M.,Wright, P.E.
Induced Fit and 'Lock and Key' Recognition of 5 S RNA by Zinc Fingers of Transcription Factor IIIA
J.Mol.Biol., 357:275-291, 2006

PubMed Abstract: Transcription factor IIIA (TFIIIA) is a Cys2His2 zinc finger protein that regulates expression of the 5 S ribosomal RNA gene by binding specifically to the internal control element. TFIIIA also functions in transport and storage of 5 S RNA by binding directly to the RNA transcript. To obtain insights into the mechanism by which TFIIIA recognizes 5 S RNA, we determined the solution structure of the middle three zinc fingers bound to the central core of 5 S RNA. Finger 4 utilizes "lock and key" recognition to bind in the widened major groove of the pre-structured RNA loop E motif. This interaction is mediated by direct hydrogen bonding interactions with bases. In contrast, recognition of loop A, a flexible junction of three helices, occurs by an induced fit mechanism that involves reorganization of the conserved CAUA motif and structuring of the finger 5-finger 6 interface to form a complementary RNA binding surface.

PubMed: 16405997
DOI: 10.1016/j.jmb.2005.12.010

実験手法

SOLUTION NMR