2HC8

Structure of the A. fulgidus CopA A-domain

2HC8 の概要

• エントリーDOI: 10.2210/pdb2hc8/pdb
• 分子名称: Cation-transporting ATPase, P-type (2 entities in total)
• 機能のキーワード: copper, atpase, copa, copb, actuator, transport protein
• 由来する生物種: Archaeoglobus fulgidus
• 細胞内の位置: Cell membrane; Multi-pass membrane protein: O29777
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11832.71

構造登録者

Sazinsky, M.H.,Agawal, S.,Arguello, J.M.,Rosenzweig, A.C. (登録日: 2006-06-15, 公開日: 2006-09-05, 最終更新日: 2024-02-14)

主引用文献

Sazinsky, M.H.,Agarwal, S.,Rosenzweig, A.C.
Structure of the Actuator Domain from the Archaeoglobus fulgidus Cu(+)-ATPase(,).
Biochemistry, 45:9949-9955, 2006

PubMed Abstract: Copper homeostasis is maintained in part by membrane-bound P(1B)-type ATPases that are found in all organisms and drive the transport of this essential, yet toxic, metal ion across cellular membranes. CopA from Archaeoglobus fulgidus is a hyperthermophilic member of this ATPase subfamily and is homologous to the human Wilson and Menkes disease ATPases. To gain insight into Cu(+)-ATPase function, the structure of the CopA actuator domain (A-domain) was determined to 1.65 A resolution. The CopA A-domain functions to couple ATP hydrolysis in the ATP binding domain (ATPBD) with structural rearrangements of critical transmembrane segments. Its fold is quite similar to that of the sarcoplasmic reticulum Ca(2+)-ATPase (SERCA1) A-domain, with the exception of an external loop region. On the basis of sequence and structural comparisons, specific residues that probably interact with the CopA ATPBD have been identified. Comparisons to the Wilson and Menkes disease A-domains reveal the presence of an additional loop that may be associated with regulatory functions in eukaryotic Cu(+)-ATPases. Finally, several mutations in the Wilson and Menkes disease ATPases occur in the A-domain, and their likely effects on function can be inferred from the CopA A-domain structure.

PubMed: 16906753
DOI: 10.1021/bi0610045

実験手法

X-RAY DIFFRACTION (1.65 Å)