2H6R

Crystal Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii

2H6R の概要

• エントリーDOI: 10.2210/pdb2h6r/pdb
• 分子名称: Triosephosphate isomerase (2 entities in total)
• 機能のキーワード: beta-alpha barrel, isomerase
• 由来する生物種: Methanocaldococcus jannaschii
• 細胞内の位置: Cytoplasm (By similarity): Q58923
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 186175.97

構造登録者

Gayathri, P.,Banerjee, M.,Vijayalakshmi, A.,Balaram, H.,Balaram, P.,Murthy, M.R.N. (登録日: 2006-06-01, 公開日: 2007-02-06, 最終更新日: 2023-10-25)

主引用文献

Gayathri, P.,Banerjee, M.,Vijayalakshmi, A.,Azeez, S.,Balaram, H.,Balaram, P.,Murthy, M.R.N.
Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii
Acta Crystallogr.,Sect.D, 63:206-220, 2007

PubMed Abstract: The crystal structure of a recombinant triosephosphate isomerase (TIM) from the archaeabacterium Methanocaldococcus jannaschii has been determined at a resolution of 2.3 A using X-ray diffraction data from a tetartohedrally twinned crystal. M. jannaschii TIM (MjTIM) is tetrameric, as suggested by solution studies and from the crystal structure, as is the case for two other structurally characterized archaeal TIMs. The archaeabacterial TIMs are shorter compared with the dimeric TIMs; the insertions in the dimeric TIMs occur in the vicinity of the tetramer interface, resulting in a hindrance to tetramerization in the dimeric TIMs. The charge distribution on the surface of the archaeal TIMs also facilitates tetramerization. Analysis of the barrel interactions in TIMs suggests that these interactions are unlikely to account for the thermal stability of the archaeal TIMs. A novelty of the unliganded structure of MjTIM is the complete absence of electron density for the loop 6 residues. The disorder of this loop could be ascribed to a missing salt bridge between residues at the N- and C-terminal ends of the loop in MjTIM.

PubMed: 17242514
DOI: 10.1107/S0907444906046488

実験手法

X-RAY DIFFRACTION (2.3 Å)