2H6P

Crystal structure of HLA-B*3501 presenting the human cytochrome P450 derived peptide, KPIVVLHGY

2H6P の概要

• エントリーDOI: 10.2210/pdb2h6p/pdb
• 分子名称: HLA-B35, Beta-2-microglobulin, 9 mer peptide from Cytochrome P450, ... (4 entities in total)
• 機能のキーワード: immune system, gene regulation
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Secreted: P61769; Endoplasmic reticulum membrane; Peripheral membrane protein: P33260
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 44715.67

構造登録者

Archbold, J.K.,Macdonald, W.A.,Rossjohn, J. (登録日: 2006-05-31, 公開日: 2006-09-19, 最終更新日: 2024-11-06)

主引用文献

Archbold, J.K.,Macdonald, W.A.,Miles, J.J.,Brennan, R.M.,Kjer-Nielsen, L.,McCluskey, J.,Burrows, S.R.,Rossjohn, J.
Alloreactivity between disparate cognate and allogeneic pMHC-I complexes is the result of highly focused, peptide-dependent structural mimicry
J.Biol.Chem., 281:34324-34332, 2006

PubMed Abstract: Our understanding of the molecular mechanisms of T cell alloreactivity remains limited by the lack of systems for which both the T cell receptor allo- and cognate ligand are known. Here we provide evidence that a single alloreactive T cell receptor interacts with analogous structural regions of its cognate ligand, HLA-B*0801(FLRGRAYGL), as its allogeneic ligand, HLA-B*3501(KPIVVLHGY). The crystal structures of the binary peptide-major histocompatibility complexes show marked differences in the conformation of the heavy chains as well as the bound peptides. Nevertheless, both epitopes possess a prominent solvent-exposed aromatic residue at position 7 flanked by a small glycine at position 8 of the peptide determinant. Moreover, regions of close structural homology between the heavy chains of HLA B8 and HLA B35 coincided with regions that have previously been implicated in "hot spots" of T cell receptor recognition. The avidity of this human T cell receptor was also comparable for the allo- and cognate ligand, consistent with the modes of T cell receptor binding being broadly similar for these complexes. Collectively, it appears that highly focused structural mimicry against a diverse structural background provides a basis for the observed alloreactivity in this system. This cross-reactivity underpins the T cell degeneracy inherent in the limited mature T cell repertoire that must respond to a vast diversity of microbial antigens.

PubMed: 16963442
DOI: 10.1074/jbc.M606755200

実験手法

X-RAY DIFFRACTION (1.9 Å)