2H4M

Karyopherin Beta2/Transportin-M9NLS

2H4M の概要

• エントリーDOI: 10.2210/pdb2h4m/pdb
• 関連するPDBエントリー: 1QBK
• 分子名称: Transportin-1, Heterogeneous nuclear ribonucleoprotein A1 (2 entities in total)
• 機能のキーワード: heat repeat, nuclear import complex, protein transport
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cytoplasm: Q92973; Nucleus . Cytoplasm : P09651
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 206204.67

構造登録者

Chook, Y.M.,Cansizoglu, A.E. (登録日: 2006-05-24, 公開日: 2006-10-24, 最終更新日: 2024-10-09)

主引用文献

Lee, B.J.,Cansizoglu, A.E.,Louis, T.H.,Zhang, Z.,Chook, Y.M.
Rules for nuclear localization sequence recognition by karyopherin beta 2.
Cell(Cambridge,Mass.), 126:543-558, 2006

PubMed Abstract: Karyopherinbeta (Kapbeta) proteins bind nuclear localization and export signals (NLSs and NESs) to mediate nucleocytoplasmic trafficking, a process regulated by Ran GTPase through its nucleotide cycle. Diversity and complexity of signals recognized by Kap betas have prevented prediction of new Kap beta substrates. The structure of Kap beta 2 (also known as Transportin) bound to one of its substrates, the NLS of hnRNP A1, that we report here explains the mechanism of substrate displacement by Ran GTPase. Further analyses reveal three rules for NLS recognition by Kap beta 2: NLSs are structurally disordered in free substrates, have overall basic character, and possess a central hydrophobic or basic motif followed by a C-terminal R/H/KX(2-5)PY consensus sequence. We demonstrate the predictive nature of these rules by identifying NLSs in seven previously known Kap beta 2 substrates and uncovering 81 new candidate substrates, confirming five experimentally. These studies define and validate a new NLS that could not be predicted by primary sequence analysis alone.

PubMed: 16901787
DOI: 10.1016/j.cell.2006.05.049

実験手法

X-RAY DIFFRACTION (3.05 Å)