2GZD

Crystal Structure of Rab11 in Complex with Rab11-FIP2

2GZD の概要

• エントリーDOI: 10.2210/pdb2gzd/pdb
• 分子名称: Ras-related protein Rab-11A, Rab11 family-interacting protein 2, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: g protein folds, a-helical coiled coil, protein transport
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Peripheral membrane protein: P62491 Q7L804
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64864.25

構造登録者

Khan, A.R. (登録日: 2006-05-11, 公開日: 2006-08-15, 最終更新日: 2024-11-06)

主引用文献

Jagoe, W.N.,Lindsay, A.J.,Read, R.J.,McCoy, A.J.,McCaffrey, M.W.,Khan, A.R.
Crystal structure of rab11 in complex with rab11 family interacting protein 2.
Structure, 14:1273-1283, 2006

PubMed Abstract: The small GTPase Rab11 regulates the recycling of endosomes to the plasma membrane via interactions with the Rab11 family of interacting proteins (FIPs). FIPs contain a highly conserved Rab binding domain (RBD) at their C termini whose structure is unknown. Here, we have determined the crystal structure of the RBD of FIP2 in complex with Rab11(GTP) by single wavelength anomalous diffraction methods. The overall structure is a heterotetramer with dyad symmetry, arranged as a Rab11-(FIP2)2-Rab11 complex. FIP2 forms a central alpha-helical coiled coil, with both helices contributing to the Rab11 binding patch on equivalent and opposite sides of the homodimer. Switch 1 of Rab11 is embedded between the two helices, while switch 2 remains flexible and is peripherally associated with the effector. The complex reveals the structural basis for Rab11 recognition by FIPs and suggests the molecular mechanisms underlying endocytic recycling pathways.

PubMed: 16905101
DOI: 10.1016/j.str.2006.06.010

実験手法

X-RAY DIFFRACTION (2.44 Å)