2GW1

Crystal Structure of the Yeast Tom70

Summary for 2GW1

• Entry DOI: 10.2210/pdb2gw1/pdb
• Descriptor: Mitochondrial precursor proteins import receptor (2 entities in total)
• Functional Keywords: tpr, protein transport
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Cellular location: Mitochondrion outer membrane ; Single-pass membrane protein : P07213
• Total number of polymer chains: 2
• Total formula weight: 117620.42

Authors

Wu, Y.,Sha, B. (deposition date: 2006-05-03, release date: 2006-06-27, Last modification date: 2024-11-13)

Primary citation

Wu, Y.,Sha, B.
Crystal structure of yeast mitochondrial outer membrane translocon member Tom70p.
Nat.Struct.Mol.Biol., 13:589-593, 2006

PubMed Abstract: A majority of the proteins targeted to the mitochondria are transported through the translocase of the outer membrane (TOM) complex. Tom70 is a major surface receptor for mitochondrial protein precursors in the TOM complex. To investigate how Tom70 receives the mitochondrial protein precursors, we have determined the crystal structure of yeast Tom70p to 3.0 A. Tom70p forms a homodimer in the crystal. Each subunit consists primarily of tetratricopeptide repeat (TPR) motifs, which are organized into a right-handed superhelix. The TPR motifs in the N-terminal domain of Tom70p form a peptide-binding groove for the C-terminal EEVD motif of Hsp70, whereas the C-terminal domain of Tom70p contains a large pocket that may be the binding site for mitochondrial precursors. The crystal structure of Tom70p provides insights into the mechanisms of precursor transport across the mitochondrion's outer membrane.

PubMed: 16767096
DOI: 10.1038/nsmb1106

Experimental method

X-RAY DIFFRACTION (3 Å)