2GV0

The structure of the orthorhombic form of soft-shelled turtle lysozyme at 1.9 angstroms resolution

2GV0 の概要

• エントリーDOI: 10.2210/pdb2gv0/pdb
• 分子名称: Lysozyme C, SULFATE ION (3 entities in total)
• 機能のキーワード: lysozyme, hydrolase
• 由来する生物種: Pelodiscus sinensis (Chinese softshell turtle)
• 細胞内の位置: Secreted: Q7LZQ1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14849.76

構造登録者

Siritapetawee, J.,Thammasirirak, S.,Yuvaniyama, J.,Robinson, R.C. (登録日: 2006-05-02, 公開日: 2007-05-08, 最終更新日: 2025-01-29)

主引用文献

Siritapetawee, J.,Thammasirirak, S.,Robinson, R.C.,Yuvaniyama, J.
The 1.9 A X-ray structure of egg-white lysozyme from Taiwanese soft-shelled turtle (Trionyx Sinensis Wiegmann) exhibits structural differences from the standard chicken-type lysozyme.
J.Biochem., 145:193-198, 2009

PubMed Abstract: Lysozyme from Taiwanese soft-shelled turtle (SSTLB) has been purified from turtle egg white and crystallized. The crystals diffract X-rays beyond 2 A resolution and belong to the orthorhombic P2(1)2(1)2(1) space group containing one molecule per asymmetric unit. The structure was elucidated using pheasant egg-white lysozyme as the molecular replacement search template. The overall structure of SSTLB is very similar to that of hen egg-white lysozyme (HEWL). Nevertheless, Pro104 in the substrate subsite A and other amino acids in the substrate subsites E and F differ from those of HEWL. These substitutions result in local conformational differences in the substrate binding sites of the two enzymes, effecting substrate binding and transglycosylation efficiency, translating into differing ranges of products.

PubMed: 19029145
DOI: 10.1093/jb/mvn156

実験手法

X-RAY DIFFRACTION (1.9 Å)