2GUZ

Structure of the Tim14-Tim16 complex of the mitochondrial protein import motor

2GUZ の概要

• エントリーDOI: 10.2210/pdb2guz/pdb
• 関連するPDBエントリー: 1HDJ 1XBL
• 分子名称: Mitochondrial import inner membrane translocase subunit TIM14, Mitochondrial import inner membrane translocase subunit TIM16, CITRATE ANION, ... (4 entities in total)
• 機能のキーワード: dnaj-fold, chaperone, protein transport
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Mitochondrion inner membrane ; Single-pass membrane protein : Q07914; Mitochondrion inner membrane ; Peripheral membrane protein : P42949
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 126701.86

構造登録者

Mokranjac, D.,Bourenkov, G.,Hell, K.,Neupert, W.,Groll, M. (登録日: 2006-05-02, 公開日: 2006-10-03, 最終更新日: 2024-02-14)

主引用文献

Mokranjac, D.,Bourenkov, G.,Hell, K.,Neupert, W.,Groll, M.
Structure and function of Tim14 and Tim16, the J and J-like components of the mitochondrial protein import motor.
Embo J., 25:4675-4685, 2006

PubMed Abstract: The import motor of the mitochondrial translocase of the inner membrane (TIM23) mediates the ATP-dependent translocation of preproteins into the mitochondrial matrix by cycles of binding to and release from mtHsp70. An essential step of this process is the stimulation of the ATPase activity of mtHsp70 performed by the J cochaperone Tim14. Tim14 forms a complex with the J-like protein Tim16. The crystal structure of this complex shows that the conserved domains of the two proteins have virtually identical folds but completely different surfaces enabling them to perform different functions. The Tim14-Tim16 dimer reveals a previously undescribed arrangement of J and J-like domains. Mutations that destroy the complex between Tim14 and Tim16 are lethal demonstrating that complex formation is an essential requirement for the viability of cells. We further demonstrate tight regulation of the cochaperone activity of Tim14 by Tim16. The first crystal structure of a J domain in complex with a regulatory protein provides new insights into the function of the mitochondrial TIM23 translocase and the Hsp70 chaperone system in general.

PubMed: 16977310
DOI: 10.1038/sj.emboj.7601334

実験手法

X-RAY DIFFRACTION (2 Å)