2GPC

The crystal structure of the enzyme Fe-superoxide dismutase from Trypanosoma cruzi

2GPC の概要

• エントリーDOI: 10.2210/pdb2gpc/pdb
• 関連するPDBエントリー: 2GOJ
• 分子名称: iron superoxide dismutase, FE (II) ION, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: alpha+beta structure, oxidoreductase
• 由来する生物種: Trypanosoma cruzi
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43895.05

構造登録者

Navarro, M.V.A.S.,Garratt, R.C. (登録日: 2006-04-17, 公開日: 2007-08-28, 最終更新日: 2023-11-15)

主引用文献

Bachega, J.F.,Navarro, M.V.,Bleicher, L.,Bortoleto-Bugs, R.K.,Dive, D.,Hoffmann, P.,Viscogliosi, E.,Garratt, R.C.
Systematic structural studies of iron superoxide dismutases from human parasites and a statistical coupling analysis of metal binding specificity
Proteins, 77:26-37, 2009

PubMed Abstract: Superoxide dismutases (SODs) are a crucial class of enzymes in the combat against intracellular free radical damage. They eliminate superoxide radicals by converting them into hydrogen peroxide and oxygen. In spite of their very different life cycles and infection strategies, the human parasites Plasmodium falciparum, Trypanosoma cruzi and Trypanosoma brucei are known to be sensitive to oxidative stress. Thus the parasite Fe-SODs have become attractive targets for novel drug development. Here we report the crystal structures of FeSODs from the trypanosomes T. brucei at 2.0 A and T. cruzi at 1.9 A resolution, and that from P. falciparum at a higher resolution (2.0 A) to that previously reported. The homodimeric enzymes are compared to the related human MnSOD with particular attention to structural aspects which are relevant for drug design. Although the structures possess a very similar overall fold, differences between the enzymes at the entrance to the channel which leads to the active site could be identified. These lead to a slightly broader and more positively charged cavity in the parasite enzymes. Furthermore, a statistical coupling analysis (SCA) for the whole Fe/MnSOD family reveals different patterns of residue coupling for Mn and Fe SODs, as well as for the dimeric and tetrameric states. In both cases, the statistically coupled residues lie adjacent to the conserved core surrounding the metal center and may be expected to be responsible for its fine tuning, leading to metal ion specificity.

PubMed: 19384994
DOI: 10.1002/prot.22412

実験手法

X-RAY DIFFRACTION (1.9 Å)