2GM5
An activated, truncated gamma-delta resolvase tetramer
Summary for 2GM5
Entry DOI | 10.2210/pdb2gm5/pdb |
Related | 1GDT 1ZR2 1ZR4 2GM4 2RSL |
Descriptor | Transposon gamma-delta resolvase (2 entities in total) |
Functional Keywords | gamma delta resolvase, site specific recombination, recombination |
Biological source | Escherichia coli |
Total number of polymer chains | 4 |
Total formula weight | 62769.63 |
Authors | Kamtekar, S.,Ho, R.S.,Li, W.,Steitz, T.A. (deposition date: 2006-04-05, release date: 2006-06-27, Last modification date: 2024-10-30) |
Primary citation | Kamtekar, S.,Ho, R.S.,Cocco, M.J.,Li, W.,Wenwieser, S.V.,Boocock, M.R.,Grindley, N.D.,Steitz, T.A. Implications of structures of synaptic tetramers of gamma delta resolvase for the mechanism of recombination. Proc.Natl.Acad.Sci.Usa, 103:10642-10647, 2006 Cited by PubMed Abstract: The structures of two mutants of the site-specific recombinase, gammadelta resolvase, that form activated tetramers have been determined. One, at 3.5-A resolution, forms a synaptic intermediate of resolvase that is covalently linked to two cleaved DNAs, whereas the other is of an unliganded structure determined at 2.1-A resolution. Comparisons of the four known tetrameric resolvase structures show that the subunits interact through the formation of a common core of four helices. The N-terminal halves of these helices superimpose well on each other, whereas the orientations of their C termini are more variable. The catalytic domains of resolvase in the unliganded structure are arranged asymmetrically, demonstrating that their positions can move substantially while preserving the four-helix core that forms the tetramer. These results suggest that the precleavage synaptic tetramer of gammadelta resolvase, whose structure is not known, may be formed by a similar four-helix core, but differ in the relative orientations of its catalytic and DNA-binding domains. PubMed: 16807292DOI: 10.1073/pnas.0604062103 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
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