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2GM5

An activated, truncated gamma-delta resolvase tetramer

Summary for 2GM5
Entry DOI10.2210/pdb2gm5/pdb
Related1GDT 1ZR2 1ZR4 2GM4 2RSL
DescriptorTransposon gamma-delta resolvase (2 entities in total)
Functional Keywordsgamma delta resolvase, site specific recombination, recombination
Biological sourceEscherichia coli
Total number of polymer chains4
Total formula weight62769.63
Authors
Kamtekar, S.,Ho, R.S.,Li, W.,Steitz, T.A. (deposition date: 2006-04-05, release date: 2006-06-27, Last modification date: 2024-10-30)
Primary citationKamtekar, S.,Ho, R.S.,Cocco, M.J.,Li, W.,Wenwieser, S.V.,Boocock, M.R.,Grindley, N.D.,Steitz, T.A.
Implications of structures of synaptic tetramers of gamma delta resolvase for the mechanism of recombination.
Proc.Natl.Acad.Sci.Usa, 103:10642-10647, 2006
Cited by
PubMed Abstract: The structures of two mutants of the site-specific recombinase, gammadelta resolvase, that form activated tetramers have been determined. One, at 3.5-A resolution, forms a synaptic intermediate of resolvase that is covalently linked to two cleaved DNAs, whereas the other is of an unliganded structure determined at 2.1-A resolution. Comparisons of the four known tetrameric resolvase structures show that the subunits interact through the formation of a common core of four helices. The N-terminal halves of these helices superimpose well on each other, whereas the orientations of their C termini are more variable. The catalytic domains of resolvase in the unliganded structure are arranged asymmetrically, demonstrating that their positions can move substantially while preserving the four-helix core that forms the tetramer. These results suggest that the precleavage synaptic tetramer of gammadelta resolvase, whose structure is not known, may be formed by a similar four-helix core, but differ in the relative orientations of its catalytic and DNA-binding domains.
PubMed: 16807292
DOI: 10.1073/pnas.0604062103
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.1 Å)
Structure validation

243531

數據於2025-10-22公開中

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