2GIS

Structure of the S-adenosylmethionine riboswitch mRNA regulatory element

2GIS の概要

• エントリーDOI: 10.2210/pdb2gis/pdb
• 分子名称: SAM-I riboswitch, MAGNESIUM ION, IRIDIUM HEXAMMINE ION, ... (5 entities in total)
• 機能のキーワード: mrna, riboswitch, s-adenosylmethionine, sam, rna-ligand complex, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32177.97

構造登録者

Montange, R.K.,Batey, R.T. (登録日: 2006-03-29, 公開日: 2006-07-04, 最終更新日: 2024-02-14)

主引用文献

Montange, R.K.,Batey, R.T.
Structure of the S-adenosylmethionine riboswitch regulatory mRNA element.
Nature, 441:1172-1175, 2006

PubMed Abstract: Riboswitches are cis-acting genetic regulatory elements found in the 5'-untranslated regions of messenger RNAs that control gene expression through their ability to bind small molecule metabolites directly. Regulation occurs through the interplay of two domains of the RNA: an aptamer domain that responds to intracellular metabolite concentrations and an expression platform that uses two mutually exclusive secondary structures to direct a decision-making process. In Gram-positive bacteria such as Bacillus species, riboswitches control the expression of more than 2% of all genes through their ability to respond to a diverse set of metabolites including amino acids, nucleobases and protein cofactors. Here we report the 2.9-angstroms resolution crystal structure of an S-adenosylmethionine (SAM)-responsive riboswitch from Thermoanaerobacter tengcongensis complexed with S-adenosylmethionine, an RNA element that controls the expression of several genes involved in sulphur and methionine metabolism. This RNA folds into a complex three-dimensional architecture that recognizes almost every functional group of the ligand through a combination of direct and indirect readout mechanisms. Ligand binding induces the formation of a series of tertiary interactions with one of the helices, serving as a communication link between the aptamer and expression platform domains.

PubMed: 16810258
DOI: 10.1038/nature04819

実験手法

X-RAY DIFFRACTION (2.9 Å)