2GIM

1.6 Angstrom structure of plastocyanin from Anabaena variabilis

2GIM の概要

• エントリーDOI: 10.2210/pdb2gim/pdb
• 分子名称: Plastocyanin, COPPER (II) ION (3 entities in total)
• 機能のキーワード: beta sheet, cu, helix, electron transport
• 由来する生物種: Anabaena variabilis
• 細胞内の位置: Cellular thylakoid membrane ; Peripheral membrane protein ; Lumenal side : P0C178
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22622.77

構造登録者

Schmidt, L.,Harris, P.,Christensen, H.E.M. (登録日: 2006-03-29, 公開日: 2006-04-11, 最終更新日: 2023-08-30)

主引用文献

Schmidt, L.,Christensen, H.E.,Harris, P.
Structure of plastocyanin from the cyanobacterium Anabaena variabilis.
ACTA CRYSTALLOGR.,SECT.D, 62:1022-1029, 2006

PubMed Abstract: Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in Escherichia coli and purified. Plate-like crystals were obtained by crystallization in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized protein was solved to a resolution of 1.6 Angstrom using plastocyanin from Phormidium laminosum as a search model. Two molecules were found in the asymmetric unit. The electrostatic surface of the basic protein showed a large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged patches. The copper ion was found to be relatively mobile and there were two distinct conformations of His61.

PubMed: 16929103
DOI: 10.1107/S0907444906023638

実験手法

X-RAY DIFFRACTION (1.6 Å)