2GFI

Crystal structure of the phytase from D. castellii at 2.3 A

2GFI の概要

• エントリーDOI: 10.2210/pdb2gfi/pdb
• 分子名称: phytase, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: 3-phytase, hydrolase
• 由来する生物種: Debaryomyces castellii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 103233.82

構造登録者

Hoh, F. (登録日: 2006-03-22, 公開日: 2007-03-27, 最終更新日: 2024-10-30)

主引用文献

Ragon, M.,Hoh, F.,Aumelas, A.,Chiche, L.,Moulin, G.,Boze, H.
Structure of Debaryomyces castellii CBS 2923 phytase.
Acta Crystallogr.,Sect.F, 65:321-326, 2009

PubMed Abstract: Phytate (myo-inositol hexakisphosphate) is the primary storage form of phosphate in seeds and legumes (Reddy et al., 1982). Phytases are phosphatases that hydrolyze phytate to less phosphorylated myo-inositol derivatives and inorganic phosphate. The crystal structure of phytase from Debaryomyces castellii has been determined at 2.3 A resolution. The crystals belonged to space group P6(5)22, with unit-cell parameters a = 121.65, c = 332.24 A. The structure was solved by molecular replacement and refined to a final R factor of 15.7% (R(free) = 20.9%). The final model consists of a dimer (with two monomers of 458 residues), five NAG molecules and 628 water molecules.

PubMed: 19342770
DOI: 10.1107/S1744309109008653

実験手法

X-RAY DIFFRACTION (2.29 Å)