2GEW

Atomic resolution structure of cholesterol oxidase @ pH 9.0 (Streptomyces sp. SA-COO)

2GEW の概要

• エントリーDOI: 10.2210/pdb2gew/pdb
• 分子名称: Cholesterol oxidase, SULFATE ION, FLAVIN-ADENINE DINUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: flavoenzyme, steroid metabolism, oxidoreductase, atomic resolution, hydrogen bond
• 由来する生物種: Streptomyces sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55883.29

構造登録者

Lyubimov, A.Y.,Vrielink, A. (登録日: 2006-03-20, 公開日: 2006-05-02, 最終更新日: 2023-08-30)

主引用文献

Lyubimov, A.Y.,Lario, P.I.,Moustafa, I.,Vrielink, A.
Atomic resolution crystallography reveals how changes in pH shape the protein microenvironment.
Nat.Chem.Biol., 2:259-264, 2006

PubMed Abstract: Hydrogen atoms are a vital component of enzyme structure and function. In recent years, atomic resolution crystallography (>or=1.2 A) has been successfully used to investigate the role of the hydrogen atom in enzymatic catalysis. Here, atomic resolution crystallography was used to study the effect of pH on cholesterol oxidase from Streptomyces sp., a flavoenzyme oxidoreductase. Crystallographic observations of the anionic oxidized flavin cofactor at basic pH are consistent with the UV-visible absorption profile of the enzyme and readily explain the reversible pH-dependent loss of oxidation activity. Furthermore, a hydrogen atom, positioned at an unusually short distance from the main chain carbonyl oxygen of Met122 at high pH, was observed, suggesting a previously unknown mechanism of cofactor stabilization. This study shows how a redox active site responds to changes in the enzyme's environment and how these changes are able to influence the mechanism of enzymatic catalysis.

PubMed: 16604066
DOI: 10.1038/nchembio784

実験手法

X-RAY DIFFRACTION (0.97 Å)