2GA4

Stx2 with adenine

2GA4 の概要

• エントリーDOI: 10.2210/pdb2ga4/pdb
• 関連するPDBエントリー: 1R4P 1R4Q
• 分子名称: Shiga-like toxin II subunit A, Shiga-like toxin II subunit B, SODIUM ION, ... (8 entities in total)
• 機能のキーワード: ab5-toxin, toxin
• 由来する生物種: Enterobacteria phage 933W; 詳細
• 細胞内の位置: Secreted: P09385 P09386
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 73698.41

構造登録者

Fraser, M.E. (登録日: 2006-03-07, 公開日: 2006-07-11, 最終更新日: 2024-10-30)

主引用文献

Fraser, M.E.,Cherney, M.M.,Marcato, P.,Mulvey, G.L.,Armstrong, G.D.,James, M.N.
Binding of adenine to Stx2, the protein toxin from Escherichia coli O157:H7.
Acta Crystallogr.,Sect.F, 62:627-630, 2006

PubMed Abstract: Stx2 is a protein toxin whose catalytic subunit acts as an N-glycosidase to depurinate a specific adenine base from 28S rRNA. In the holotoxin, the catalytic portion, A1, is linked to the rest of the A subunit, A2, and A2 interacts with the pentameric ring formed by the five B subunits. In order to test whether the holotoxin is active as an N-glycosidase, Stx2 was crystallized in the presence of adenosine and adenine. The crystals diffracted to approximately 1.8 angstroms and showed clear electron density for adenine in the active site. Adenosine had been cleaved, proving that Stx2 is an active N-glycosidase. While the holotoxin is active against small substrates, it would be expected that the B subunits would interfere with the binding of the 28S rRNA.

PubMed: 16820678
DOI: 10.1107/S1744309106021968

実験手法

X-RAY DIFFRACTION (1.8 Å)