2G9T

Crystal structure of the SARS coronavirus nsp10 at 2.1A

2G9T の概要

• エントリーDOI: 10.2210/pdb2g9t/pdb
• 関連するPDBエントリー: 2GA6
• 分子名称: orf1a polyprotein, ZINC ION (3 entities in total)
• 機能のキーワード: sars, nsp10, viral protein
• 由来する生物種: Severe acute respiratory syndrome-related coronavirus
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 390460.20

構造登録者

Su, D.,Lou, Z.,Yang, H.,Sun, F.,Rao, Z. (登録日: 2006-03-07, 公開日: 2006-08-15, 最終更新日: 2024-03-13)

主引用文献

Su, D.,Lou, Z.,Sun, F.,Zhai, Y.,Yang, H.,Zhang, R.,Joachimiak, A.,Zhang, X.C.,Bartlam, M.,Rao, Z.
Dodecamer Structure of Severe Acute Respiratory Syndrome Coronavirus Nonstructural Protein nsp10
J.Virol., 80:7902-7908, 2006

PubMed Abstract: The severe acute respiratory syndrome coronavirus (SARS-CoV) nonstructural proteins nsp1 to nsp16 have been implicated by genetic analysis in the assembly of a functional replication/transcription complex. We report the crystal structure of nsp10 from SARS-CoV at 2.1-A resolution. The nsp10 structure has a novel fold, and 12 identical subunits assemble to form a unique spherical dodecameric architecture. Two zinc fingers have been identified from the nsp10 monomer structure with the sequence motifs C-(X)2-C-(X)5-H-(X)6-C and C-(X)2-C-(X)7-C-(X)-C. The nsp10 crystal structure is the first of a new class of zinc finger protein three-dimensional structures to be revealed experimentally. The zinc finger sequence motifs are conserved among all three coronavirus antigenic groups, implicating an essential function for nsp10 in all coronaviruses. Based on the structure, we propose that nsp10 is a transcription factor for coronavirus replication/transcription.

PubMed: 16873247
DOI: 10.1128/JVI.00483-06

実験手法

X-RAY DIFFRACTION (2.1 Å)