2G7E

The 1.6 A crystal structure of Vibrio cholerae extracellular endonuclease I

2G7E の概要

• エントリーDOI: 10.2210/pdb2g7e/pdb
• 関連するPDBエントリー: 2G7F
• 分子名称: Endonuclease I, CHLORIDE ION (3 entities in total)
• 機能のキーワード: vibrio cholerae nuclease; enda; endonuclease i;buried chloride, hydrolase
• 由来する生物種: Vibrio cholerae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24565.88

構造登録者

Altermark, B.,Smalaas, A.O.,Willassen, N.P.,Helland, R. (登録日: 2006-02-28, 公開日: 2006-10-31, 最終更新日: 2024-10-30)

主引用文献

Altermark, B.,Smalas, A.O.,Willassen, N.P.,Helland, R.
The structure of Vibrio cholerae extracellular endonuclease I reveals the presence of a buried chloride ion.
Acta Crystallogr.,Sect.D, 62:1387-1391, 2006

PubMed Abstract: The crystal structure of a periplasmic/extracellular endonuclease from Vibrio cholerae has been solved at low and at neutral pH. Crystals grown at pH 4.6 and 6.9 diffracted to 1.6 A (on BM01A at the ESRF) and 1.95 A (on a rotating-anode generator), respectively. The structures of the endonuclease were compared with the structure of a homologous enzyme in V. vulnificus. The structures of the V. cholerae enzyme at different pH values are essentially identical to each other and to the V. vulnificus enzyme. However, interesting features were observed in the solvent structures. Both V. cholerae structures reveal the presence of a chloride ion completely buried within the core of the protein, with the nearest solvent molecule approximately 7 A away. Magnesium, which is essential for catalysis, is present in the structure at neutral pH, but is absent at low pH, and may partly explain the inactivity of the enzyme at lower pH.

PubMed: 17057343
DOI: 10.1107/S0907444906034196

実験手法

X-RAY DIFFRACTION (1.6 Å)